Vahidi R, Safi S, Farsinejad A, Panahi N
Islamic Azad University Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch Tehran Iran.
Islamic Azad University Department of Pathobiology, Faculty of Specialized Veterinary Sciences, Science and Research Branch Tehran Iran safishahab@yahoo.com.
Cell Mol Biol (Noisy-le-grand). 2015 Oct 16;61(5):22-8.
Celecoxib and citrate have been shown to possess antitumor activity in a variety of cancer cells. However, the antitumor activities of these agents in canine mammary tumors have not been well demonstrated. The aim of our study was to investigate the apoptotic and antiproliferative effects of citrate and celecoxib, individually and in combination, on canine mammary tumor cell line CF41—Mg. MTT assay was performed to determine cell viability, and Annexin—PI test was performed to evaluate apoptosis induction. MTT assay results revealed that compared with the control groups, treatment groups, as both single and combined treatments, showed significant inhibition of tumor growth in a dose—dependent manner. IC50 concentrations of citrate and celecoxib were defined 26mM and 22μM, respectively. In another set of experiment, significant increase in cell apoptosis was observed at IC50 concentrations of citrate and celecoxib after 48h incubation. In spite of that, simultaneous treatment of cells with citrate and celecoxib eventuated with meaningful toxicity augmentation and induction of apoptosis at lower concentrations. Also necrotic cells were decreased by coadministration of the two agents. In conclusion, the present study indicates significant cytotoxic and apoptotic effects of citrate and celecoxib coadministration on CF41—Mg cells, and proposes new strategies for counteracting cancer cells proliferation and overcoming chemo resistance.
塞来昔布和柠檬酸盐已被证明在多种癌细胞中具有抗肿瘤活性。然而，这些药物在犬乳腺肿瘤中的抗肿瘤活性尚未得到充分证实。我们研究的目的是调查柠檬酸盐和塞来昔布单独及联合使用对犬乳腺肿瘤细胞系CF41—Mg的凋亡和抗增殖作用。进行MTT试验以确定细胞活力，并进行膜联蛋白—碘化丙啶检测以评估凋亡诱导情况。MTT试验结果显示，与对照组相比，作为单一治疗和联合治疗的治疗组均以剂量依赖性方式显著抑制肿瘤生长。柠檬酸盐和塞来昔布的IC50浓度分别确定为26mM和22μM。在另一组实验中，孵育48小时后，在柠檬酸盐和塞来昔布的IC50浓度下观察到细胞凋亡显著增加。尽管如此，柠檬酸盐和塞来昔布同时处理细胞会导致有意义的毒性增强，并在较低浓度下诱导凋亡。同时，两种药物共同给药可减少坏死细胞。总之，本研究表明柠檬酸盐和塞来昔布联合给药对CF41—Mg细胞具有显著的细胞毒性和凋亡作用，并提出了对抗癌细胞增殖和克服化疗耐药性的新策略。
