Department of Stem Cell Regulation, Medical Research Institute, Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan.
Department of Nutritional Sciences, Faculty of Nutritional Sciences, Nakamura Gakuen University, 5-7-1 Befu, Jonan-ku, Fukuoka 814-0198, Japan.
Cells. 2024 Nov 7;13(22):1840. doi: 10.3390/cells13221840.
During mouse development, hematopoietic cells first form in the extraembryonic tissue yolk sac. Hematopoietic stem cells (HSCs), which retain their ability to differentiate into hematopoietic cells for a long time, form intra-aortic hematopoietic cell clusters (IAHCs) in the dorsal aorta at midgestation. These IAHCs emerge from the hemogenic endothelium, which is the common progenitor of hematopoietic cells and endothelial cells. HSCs expand in the fetal liver, and finally migrate to the bone marrow (BM) during the peripartum period. IAHCs are absent in the dorsal aorta in mice deficient in transcription factors such as Runx-1, GATA2, and c-Myb that are essential for definitive hematopoiesis. In this review, we focus on the transcription factor Sry-related high mobility group (HMG)-box (Sox) F family of proteins that is known to regulate hematopoiesis in the hemogenic endothelium and IAHCs. The SoxF family is composed of Sox7, Sox17, and Sox18, and they all have the HMG box, which has a DNA-binding ability, and a transcriptional activation domain. Here, we describe the functional and phenotypic properties of SoxF family members, with a particular emphasis on Sox17, which is the most involved in hematopoiesis in the fetal stages considering that enhanced expression of Sox17 in hemogenic endothelial cells and IAHCs leads to the production and maintenance of HSCs. We also discuss SoxF-inducing signaling pathways.
在小鼠发育过程中，造血细胞最初在胚外组织卵黄囊中形成。造血干细胞（HSCs）能够长期保持分化为造血细胞的能力，在中孕期形成主动脉内造血细胞簇（IAHCs）位于背主动脉。这些 IAHCs 起源于造血细胞和内皮细胞的共同祖细胞——造血内皮细胞。HSCs 在胎儿肝脏中扩增，最终在围产期迁移到骨髓（BM）。在缺乏对确定造血至关重要的转录因子（如 Runx-1、GATA2 和 c-Myb）的小鼠中，背主动脉中不存在 IAHCs。在这篇综述中，我们重点介绍了已知在造血内皮细胞和 IAHCs 中调节造血的 Sry 相关高迁移率族（HMG）-盒（Sox）F 家族蛋白的转录因子。SoxF 家族由 Sox7、Sox17 和 Sox18 组成，它们都具有 HMG 盒，具有 DNA 结合能力和转录激活结构域。在这里，我们描述了 SoxF 家族成员的功能和表型特性，特别强调 Sox17，因为考虑到 Sox17 在造血内皮细胞和 IAHCs 中的高表达会导致 HSCs 的产生和维持，Sox17 在胎儿期的造血中最为重要。我们还讨论了 SoxF 诱导的信号通路。
