Kowalewska Paulina M, Margetts Peter J, Fox-Robichaud Alison E
Medical Sciences Graduate Program, McMaster University, Hamilton, ON, Canada.
Division of Nephrology, St. Joseph's Hospital Department of Medicine, McMaster University, Hamilton, ON, Canada.
Perit Dial Int. 2016 Jan-Feb;36(1):7-15. doi: 10.3747/pdi.2014.00211. Epub 2015 Oct 16.
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The objective of this study was to examine the effects of a conventional dialysis solution and peritoneal catheter on leukocyte-endothelial cell interactions in the microcirculation of the parietal peritoneum in a subacute peritoneal dialysis (PD) mouse model. ♦
An intraperitoneal (IP) catheter with a subcutaneous injection port was implanted into mice and, after a 2-week healing period, the animals were injected daily for 6 weeks with a 2.5% dextrose solution. Intravital microscopy (IVM) of the parietal peritoneum microcirculation was performed 4 hours after the last injection of the dialysis solution. Leukocyte-endothelial cell interactions were quantified and compared with catheterized controls without dialysis treatment and naïve mice. ♦
The number of rolling and extravascular leukocytes along with peritoneal fibrosis and neovascularization were significantly increased in the catheterized animals compared with naïve mice but did not significantly differ between the 2 groups of catheterized animals with sham injections or dialysis solution treatment. ♦
The peritoneal catheter implant increased leukocyte rolling and extravasation, peritoneal fibrosis and vascularization in the parietal peritoneum independently from the dialysis solution treatment.
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本研究的目的是在亚急性腹膜透析（PD）小鼠模型中，研究传统透析液和腹膜导管对壁层腹膜微循环中白细胞与内皮细胞相互作用的影响。♦
将带有皮下注射端口的腹腔（IP）导管植入小鼠体内，在2周的愈合期后，每天给动物注射2.5%的葡萄糖溶液，持续6周。在最后一次注射透析液4小时后，对壁层腹膜微循环进行活体显微镜检查（IVM）。对白细胞与内皮细胞的相互作用进行定量，并与未进行透析治疗的导管插入对照组和未处理的小鼠进行比较。♦
与未处理的小鼠相比，导管插入组动物的滚动和血管外白细胞数量以及腹膜纤维化和新生血管形成显著增加，但在接受假注射或透析液治疗的两组导管插入动物之间没有显著差异。♦
腹膜导管植入独立于透析液治疗，增加了壁层腹膜中的白细胞滚动和渗出、腹膜纤维化和血管形成。
