Terahara Takeshi, Yamada Katsuhisa, Nakayama Jiro, Igarashi Yasuhiro, Kobayashi Takeshi, Imada Chiaki
Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan.
Graduate School of Marine Science of Technology, Tokyo University of Marine Science and Technology, 4-5-7, Konan, Minato-ku, Tokyo 108-8477, Japan; DHC Corporation, 2-7-1, Minami-azabu, Minato-ku, Tokyo 106-8571, Japan.
Gene. 2016 Feb 1;576(2 Pt 1):696-700. doi: 10.1016/j.gene.2015.10.027. Epub 2015 Oct 22.
The aim of the present study was to investigate the bacterial community structures of deep-sea water (DSW) and surface seawater (SSW) samples in Japan by molecular biological techniques. DGGE analyses and pyrosequencing analysis revealed that bacterial community structures of DSW were diverse and differed from those of SSW. This is the first report on the horizontal variation of bacterial community structures of DSW throughout Japan. In addition, pyrosequencing analysis revealed that the number of phyla in DSW was larger than that in SSW, and specific phyla, such as Firmicutes and Planctomycetes, were characterized by a higher proportion of the bacterial community structure in DSW than in SSW. Taken together, these results indicate that a variety of bacteria that are specifically adapted to the DSW environments can be expected to be found in DSW, and DSW would thus be a potential resource for novel or unique microorganisms and compounds.
本研究的目的是通过分子生物学技术调查日本深海海水（DSW）和表层海水（SSW）样本的细菌群落结构。变性梯度凝胶电泳（DGGE）分析和焦磷酸测序分析表明，DSW的细菌群落结构多样，与SSW不同。这是关于日本各地DSW细菌群落结构水平变化的首次报告。此外，焦磷酸测序分析表明，DSW中的门数量比SSW中的多，并且特定的门，如厚壁菌门和浮霉菌门，在DSW细菌群落结构中的比例高于SSW。综上所述，这些结果表明，有望在DSW中发现各种特别适应DSW环境的细菌，因此DSW将是新型或独特微生物及化合物的潜在资源。
