用于研究海洋浮游细菌群落的不同变性梯度凝胶电泳引物组的比较
Comparison of different denaturing gradient gel electrophoresis primer sets for the study of marine bacterioplankton communities.
作者信息
Sánchez Olga, Gasol Josep M, Massana Ramon, Mas Jordi, Pedrós-Alió Carlos
机构信息
Departament de Genètica i Microbiologia, Facultat de Ciències, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
出版信息
Appl Environ Microbiol. 2007 Sep;73(18):5962-7. doi: 10.1128/AEM.00817-07. Epub 2007 Jul 27.
Abstract
An annual seasonal cycle of composition of a bacterioplankton community in an oligotrophic coastal system was studied by denaturing gradient gel electrophoresis (DGGE) using five different primer sets. Analysis of DGGE fingerprints showed that primer set 357fGC-907rM grouped samples according to seasons. Additionally, we used the set of 16S rRNA genes archived in the RDPII database to check the percentage of perfect matches of each primer for the most abundant bacterial groups inhabiting coastal plankton communities. Overall, primer set 357fGC-907rM was the most suitable for the routine use of PCR-DGGE analyses in this environment.
摘要
利用五种不同的引物组，通过变性梯度凝胶电泳（DGGE）研究了贫营养沿海系统中浮游细菌群落组成的年度季节性循环。对DGGE指纹图谱的分析表明，引物组357fGC-907rM可根据季节对样本进行分类。此外，我们使用RDPII数据库中存档的16S rRNA基因集，来检查每种引物与栖息在沿海浮游生物群落中最丰富细菌类群的完美匹配百分比。总体而言，引物组357fGC-907rM最适合在这种环境下常规用于PCR-DGGE分析。
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