槲皮素可保护HCT116细胞免受敌敌畏诱导的氧化应激和细胞凋亡。
Quercetin protects HCT116 cells from Dichlorvos-induced oxidative stress and apoptosis.
作者信息
Ben Salem Intidhar, Boussabbeh Manel, Graiet Imen, Rhouma Asma, Bacha Hassen, Abid Essefi Salwa
机构信息
Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, Rue Avicenne, 5000, Monastir, Tunisia.
Faculty of Sciences of Bizerte, Carthage University, Tunis, Tunisia.
出版信息
Cell Stress Chaperones. 2016 Jan;21(1):179-186. doi: 10.1007/s12192-015-0651-7.
The present study was designed to assess the possible protective effects of Quercetin (QUER), a flavonoid with well-known pharmacological effects, against Dichlorvos (DDVP)-induced toxicity in vitro using HCT116 cells. The cytotoxicity was monitored by cell viability, reactive oxygen species (ROS) generation, anti-oxidant enzyme activities, malondialdehyde (MDA) production, and DNA fragmentation. The apoptosis was assessed through the measurement of the mitochondrial transmembrane potential (ΔΨm) and caspase activation. The results indicated that pretreatment of HCT116 cells with QUER, 2 h prior to DDVP exposure, significantly decreased the DDVP-induced cell death, inhibited the ROS generation, modulated the activities of catalase (CAT) and superoxide dismutase (SOD), and reduced the MDA level. The reductions in mitochondrial membrane potential, DNA fragmentation, and caspase activation were also attenuated by QUER. These findings suggest that dietary QUER can protect HCT116 cells against DDVP-induced oxidative stress and apoptosis.
本研究旨在评估具有众所周知药理作用的类黄酮槲皮素（QUER）对敌敌畏（DDVP）体外诱导的HCT116细胞毒性的可能保护作用。通过细胞活力、活性氧（ROS）生成、抗氧化酶活性、丙二醛（MDA）产生和DNA片段化来监测细胞毒性。通过测量线粒体跨膜电位（ΔΨm）和半胱天冬酶激活来评估细胞凋亡。结果表明，在DDVP暴露前2小时用QUER预处理HCT116细胞，可显著降低DDVP诱导的细胞死亡，抑制ROS生成，调节过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性，并降低MDA水平。QUER还减弱了线粒体膜电位、DNA片段化和半胱天冬酶激活的降低。这些发现表明，膳食QUER可以保护HCT116细胞免受DDVP诱导的氧化应激和细胞凋亡。
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