Department of Basic Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, Hunan, China.
Molecules. 2018 Aug 12;23(8):2012. doi: 10.3390/molecules23082012.
Weanling stress and toxicosis, which are harmful to the health of pigs' intestines, are associated with oxidative stress. Quercetin (Que) is a polyphenolic compound that shows good anti-cancer, anti-inflammation and anti-oxidation effects. This study aimed to elaborate whether or not Que promotes IPEC-J2 (intestinal porcine enterocyte cells) proliferation and protects IPEC-J2 from oxidative damage. Thus, we examined the effects of Que on proliferation and H₂O₂-induced apoptosis in IPEC-J2. The results showed that Que increased IPEC-J2 viabililty, propelled cells from G1 phase into S phase and down-regulated gene levels of P27 and P21, respectively. Besides, H₂O₂-induced cell damage was alleviated by Que after different exposure times, and Que depressed apoptosis rate, reactive oxygen species (ROS) level and percentage of G1 phase cells and elevated the percentage of cells in G2 phase and S phase and mitochondrial membrane potential (Δψm) after IPEC-J2 exposure to H₂O₂. Meanwhile, Que reduced the value of Bax/Bcl-2 in H₂O₂ exposed cells. In low-degree oxidative damage cells, lipid peroxidation product malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were increased. In turn, Que could reverse the change of MDA content and SOD activity in low-degree damage cells. Nevertheless, catalase (CAT) activity was not changed in IPEC-J2 incubated with Que under low-degree damage conditions. Interestingly, relative expressive levels of the proteins claudin-1 and occludin were not altered under low-degree damage conditions, but Que could improve claudin-1 and occludin levels, slightly. This research indicates that Que can be greatly beneficial for intestinal porcine enterocyte cell proliferation and it protects intestinal porcine enterocyte cells from oxidation-induced apoptosis, and could be used as a potential feed additive for porcine intestinal health against pathogenic factor-induced oxidative damages and apoptosis.
断奶应激和中毒会对猪肠道健康造成损害，与氧化应激有关。槲皮素（Que）是一种多酚化合物，具有良好的抗癌、抗炎和抗氧化作用。本研究旨在探讨 Que 是否促进 IPEC-J2（猪肠上皮细胞）增殖并保护 IPEC-J2 免受氧化损伤。因此，我们研究了 Que 对 IPEC-J2 增殖和 H₂O₂诱导的细胞凋亡的影响。结果表明，Que 增加了 IPEC-J2 的活力，将细胞从 G1 期推进到 S 期，并分别下调了 P27 和 P21 的基因水平。此外，Que 减轻了不同暴露时间后 H₂O₂诱导的细胞损伤，降低了细胞凋亡率、活性氧（ROS）水平和 G1 期细胞比例，提高了 G2 期和 S 期细胞比例和线粒体膜电位（Δψm），同时降低了 H₂O₂ 暴露后细胞中 Bax/Bcl-2 的比值。在低度氧化损伤细胞中，脂质过氧化产物丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性增加。相反，Que 可以逆转低损伤细胞 MDA 含量和 SOD 活性的变化。然而，在低损伤条件下，Que 孵育的 IPEC-J2 中过氧化氢酶（CAT）活性没有变化。有趣的是，在低度损伤条件下，Claudin-1 和 Occludin 的相对表达水平没有改变，但 Que 可以轻微提高 Claudin-1 和 Occludin 的水平。本研究表明，Que 可以促进猪肠上皮细胞增殖，保护猪肠上皮细胞免受氧化诱导的凋亡，可作为一种潜在的饲料添加剂，用于猪肠道健康，防止病原因子诱导的氧化损伤和凋亡。
