Ma Xiaoli, Cui Weina, Liang Wenji, Huang Zhanjing
College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China.
College of Life Science, Hebei Normal University, Shijiazhuang 050024, People's Republic of China; College of Clinical Medicine, North China University of Science and Technology, Tangshan 063000, People's Republic of China.
Plant Physiol Biochem. 2015 Dec;97:187-95. doi: 10.1016/j.plaphy.2015.10.010. Epub 2015 Oct 22.
A novel salt-induced gene with unknown functions was cloned through analysis of gene expression profile of a salt-tolerant wheat mutant RH8706-49 under salt stress. The gene was named Triticum aestivum salt-related protein (TaSP) and deposited in GenBank (Accession No. KF307326). Quantitative polymerase chain reaction (qPCR) results showed that TaSP expression was induced under salt, abscisic acid (ABA), and polyethylene glycol (PEG) stresses. Subcellular localization revealed that TaSP was mainly localized in cell membrane. Overexpression of TaSP in Arabidopsis could improve salt tolerance of 35S::TaSP transgenic Arabidopsis. 35S::TaSP transgenic Arabidopsis lines after salt stress presented better physiological indexes than the control group. In the non-invasive micro-test (NMT), an evident Na(+) excretion was observed at the root tip of salt-stressed 35S::TaSP transgenic Arabidopsis. TaSP promoter was cloned, and its beta-glucuronidase (GUS) activities before and after ABA, salt, cold, heat, and salicylic acid (SA) stresses were determined. Full-length TaSP promoter contained ABA and salt response elements.
通过对耐盐小麦突变体RH8706 - 49在盐胁迫下的基因表达谱分析，克隆了一个功能未知的新型盐诱导基因。该基因被命名为普通小麦盐相关蛋白（TaSP），并保存在GenBank中（登录号：KF307326）。定量聚合酶链反应（qPCR）结果表明，TaSP的表达在盐、脱落酸（ABA）和聚乙二醇（PEG）胁迫下被诱导。亚细胞定位显示TaSP主要定位于细胞膜。在拟南芥中过表达TaSP可以提高35S::TaSP转基因拟南芥的耐盐性。盐胁迫后的35S::TaSP转基因拟南芥株系呈现出比对照组更好的生理指标。在非损伤微测技术（NMT）中，在盐胁迫的35S::TaSP转基因拟南芥根尖观察到明显的Na(+)外排。克隆了TaSP启动子，并测定了其在ABA、盐、冷、热和水杨酸（SA）胁迫前后的β - 葡萄糖醛酸酶（GUS）活性。TaSP启动子全长包含ABA和盐应答元件。
Zotero 插件