Plant Genetic Engineering Center of Hebei Province, Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China.
Genes (Basel). 2023 Aug 4;14(8):1586. doi: 10.3390/genes14081586.
Salt stress is one of the important environmental factors that inhibit the normal growth and development of plants. Plants have evolved various mechanisms, including signal transduction regulation, physiological regulation, and gene transcription regulation, to adapt to environmental stress. MicroRNAs (miRNAs) play a role in regulating mRNA expression. Nevertheless, miRNAs related to salt stress are rarely reported in bread wheat ( L.). In this study, using high-throughput sequencing, we analyzed the miRNA expression profile of wheat under salt stress. We identified 360 conserved and 859 novel miRNAs, of which 49 showed considerable changes in transcription levels after salt treatment. Among them, 25 were dramatically upregulated and 24 were downregulated. Using real-time quantitative PCR, we detected significant changes in the relative expression of miRNAs, and the results showed the same trend as the sequencing data. In the salt-treated group, miR109 had a higher expression level, while miR60 and miR202 had lower expression levels. Furthermore, 21 miRNAs with significant changes were selected from the differentially expressed miRNAs, and 1023 candidate target genes were obtained through the prediction of the website psRNATarget. Gene ontology (GO) analysis of the candidate target genes showed that the expressed miRNA may be involved in the response to biological processes, molecular functions, and cellular components. In addition, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed their important functions in RNA degradation, metabolic pathways, synthesis pathways, peroxisome, environmental adaptation, global and overview maps, and stress adaptation and the MAPK signal pathway. These findings provide a basis for further exploring the function of miRNA in wheat salt tolerance.
盐胁迫是抑制植物正常生长和发育的重要环境因素之一。植物已经进化出各种机制，包括信号转导调节、生理调节和基因转录调节，以适应环境胁迫。microRNAs（miRNAs）在调节 mRNA 表达中发挥作用。然而，在普通小麦（ L.）中，很少有报道与盐胁迫相关的 miRNAs。在这项研究中，我们使用高通量测序分析了小麦在盐胁迫下的 miRNA 表达谱。我们鉴定了 360 个保守和 859 个新的 miRNAs，其中 49 个在盐处理后转录水平发生了显著变化。其中，25 个显著上调，24 个下调。使用实时定量 PCR，我们检测到 miRNA 相对表达的显著变化，结果与测序数据的趋势相同。在盐处理组中，miR109 的表达水平较高，而 miR60 和 miR202 的表达水平较低。此外，从差异表达的 miRNAs 中选择了 21 个具有显著变化的 miRNAs，并通过 psRNATarget 网站预测获得了 1023 个候选靶基因。候选靶基因的基因本体（GO）分析表明，表达的 miRNA 可能参与了对生物过程、分子功能和细胞成分的响应。此外，京都基因与基因组百科全书（KEGG）通路分析证实了它们在 RNA 降解、代谢途径、合成途径、过氧化物酶体、环境适应、全局和概述图谱、应激适应和 MAPK 信号通路中的重要功能。这些发现为进一步探索 miRNA 在小麦耐盐性中的功能提供了依据。
