Wang Zhiguo, Kuang Ruixia, Xu Quanchen, Chen Zhenyu, Li Peng
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2015 Apr;29(4):467-71.
To explore the reaction of normal skin fibroblasts from different sites of human body to cyclic stretch.
The normal skin tissues from scapular upper back and medial side of upper arm of 3 patients were cultured in vitro. Fibroblasts of experimental group were loaded by cyclic stretch with 10% amplitude for 24, 36, and 48 hours respectively. Fibroblasts of control group were cultured without cyclic stretch. The morphologic changes were observed using inverted microscope. CCK-8 method was used to detect the proliferation of the fibroblasts. The expressions of integrin beta1 mRNA, p130Crk-associated substance (P130Cas) mRNA, transform growth factor beta1 (TGF-beta1) mRNA, and collagen type I alpha1 chain (COL1A1) mRNA were detected by real-time quantitative PCR. The protein levels of collagen type I and TGF-beta1 were detected by ELISA.
The cultured cells showed a significantly increased cell proliferation ability, and apparent orientation after the applied strain. The proliferation activity, mRNA expression levels of integrin beta1, P130Cas, and TGF-beta1, protein levels of TGF-beta1 in back skin were significantly higher than those in arm skin (P<0.05) when the fibroblasts were loaded for 36 and 48 hours, but no significant difference between back skin and arm skin at 24 hours (P>0.05). There was no significant difference in mRNA expression level of COL1A1 and protein level of collagen type I between back skin and arm skin at 24, 36, and 48 hours (P>0.05). There was no significant difference in all above indexes between back skin and arm skin in control group (P>0.05).
Fibroblasts from scapular upper back and medial side of upper arm display different reactions to cyclic stretch, which indicates that there exists site difference in the reactions of fibroblasts to cyclic stretch. It might be related with the incidence of hypertrophic scar in different sites of the body.
探讨人体不同部位正常皮肤成纤维细胞对周期性拉伸的反应。
取3例患者肩胛区上背部及上臂内侧的正常皮肤组织进行体外培养。实验组成纤维细胞分别给予10%幅度的周期性拉伸加载24、36和48小时。对照组成纤维细胞不进行周期性拉伸培养。采用倒置显微镜观察形态学变化。用CCK-8法检测成纤维细胞的增殖情况。通过实时定量PCR检测整合素β1 mRNA、p130Crk相关物质（P130Cas）mRNA、转化生长因子β1（TGF-β1）mRNA和I型胶原α1链（COL1A1）mRNA的表达。用ELISA法检测I型胶原和TGF-β1的蛋白水平。
培养的细胞在施加应变后细胞增殖能力显著增强，且出现明显的定向排列。当成纤维细胞加载36和48小时时，背部皮肤的增殖活性、整合素β1、P130Cas和TGF-β1的mRNA表达水平、TGF-β1的蛋白水平均显著高于上臂皮肤（P<0.05），但在24小时时背部皮肤与上臂皮肤之间无显著差异（P>0.05）。在24、36和48小时时，背部皮肤与上臂皮肤之间COL1A1的mRNA表达水平和I型胶原的蛋白水平无显著差异（P>0.05）。对照组背部皮肤与上臂皮肤上述各项指标之间均无显著差异（P>0.05）。
肩胛区上背部和上臂内侧的成纤维细胞对周期性拉伸表现出不同反应，这表明成纤维细胞对周期性拉伸的反应存在部位差异。这可能与身体不同部位肥厚性瘢痕的发生率有关。
