Xu Quan-Chen, Kuang Rui-Xia, Wei Shu-Qiang, Kang Qin, Wang Juan-Juan, Wang Zhi-Guo
From the *Affiliated Hospital of Qingdao University; and †The Second Affiliated Hospital of Qingdao University, Qingdao; ‡Dermatology Hospital, Rizhao; and §Department of Burn and Plastic Surgery, Affiliated Hospital of Qingdao University, Qingdao, China.
Ann Plast Surg. 2017 Nov;79(5):438-443. doi: 10.1097/SAP.0000000000001121.
Facial skin fibroblasts imposed with cyclic stretch at 10% magnitude display considerable mechanotransduction properties and biochemical reactions in our previous study. However, it is poorly understood how these shared traits are fully parallel to the common features across all fibroblasts derived from different skin-based anatomical regions in response to cyclic stretch stimulation. Thus, the purpose of this study was to evaluate the effects of various cyclic stretches on fibroblasts derived from multiple anatomical skin sites of human bodies, and the optimal stretch magnitude was defined based on the changes to cell mechanical behavior.
Fibroblasts from skin areas of the scalp, anterior chest, suprapubic, axilla, and planta were cultured and characterized in vitro. Cyclic stretch at 0%, 5%, 10%, 15%, and 20% magnitudes was imposed at a loading frequency of 0.1 Hz for 48 hours, and thereafter, the mechanical behavior and biochemical reaction of the dermal fibroblasts were analyzed.
Dermal fibroblasts from various anatomical sites preconditioned with varying cyclic stretch led to an evident increase in the cell proliferation ability, the expression of integrin β1 and p130 Crk-associated substrate messenger RNA and protein, and the productions of type I collagen and transforming growth factor β1, most importantly in a strain magnitude-dependent manner with the peak appearing in the range of 10% to 15% magnitude cyclic stretch.
These findings may facilitate the subsequent studies on the conversion of normal skin fibroblasts into hypertrophic scar cells, which should be considered in an interpretation of the mechanisms of hypertrophic scarring and skin mechanics.
在我们之前的研究中，施加10%幅度循环拉伸的面部皮肤成纤维细胞表现出相当的机械转导特性和生化反应。然而，对于这些共同特征如何完全平行于源自不同皮肤解剖区域的所有成纤维细胞在循环拉伸刺激下的共同特征，人们了解甚少。因此，本研究的目的是评估各种循环拉伸对源自人体多个皮肤解剖部位的成纤维细胞的影响，并根据细胞力学行为的变化确定最佳拉伸幅度。
对来自头皮、前胸、耻骨上、腋窝和足底皮肤区域的成纤维细胞进行体外培养和表征。以0.1 Hz的加载频率施加0%、5%、10%、15%和20%幅度的循环拉伸，持续48小时，然后分析真皮成纤维细胞的力学行为和生化反应。
用不同循环拉伸预处理的来自不同解剖部位的真皮成纤维细胞导致细胞增殖能力、整合素β1和p130 Crk相关底物信使核糖核酸及蛋白质的表达以及I型胶原蛋白和转化生长因子β-1的产生明显增加，最重要的是以应变幅度依赖性方式增加，峰值出现在10%至15%幅度的循环拉伸范围内。
这些发现可能有助于后续关于正常皮肤成纤维细胞向肥厚性瘢痕细胞转化的研究，在解释肥厚性瘢痕形成机制和皮肤力学时应予以考虑。
