Kaminker Ilia, Barnes Ryan, Han Songi
Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, USA.
Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, California, USA; Department of Chemical Engineering, University of California Santa Barbara, Santa Barbara, California, USA.
Methods Enzymol. 2015;564:457-83. doi: 10.1016/bs.mie.2015.06.040. Epub 2015 Aug 24.
Overhauser dynamic nuclear polarization (ODNP) is an emerging technique for quantifying translational water dynamics in the vicinity (<1 nm) of stable radicals that can be chemically attached to macromolecules of interest. This has led to many in-depth and enlightening studies of hydration water of biomolecules, revolving around the role of solvent dynamics in the structure and function of proteins, nucleic acids, and lipid bilayer membranes. Still to date, a complete and fully automated ODNP instrument is not commercialized. The purpose of this chapter is to share the technical know-how of the hardware, theory, measurement, and data analysis method needed to successfully utilize and disseminate the ODNP technique.
奥弗豪泽动态核极化（ODNP）是一种新兴技术，用于量化稳定自由基附近（<1 nm）的平移水动力学，这些自由基可以化学连接到感兴趣的大分子上。这引发了许多关于生物分子水化水的深入且有启发性的研究，围绕溶剂动力学在蛋白质、核酸和脂质双层膜的结构与功能中的作用展开。然而迄今为止，完整且完全自动化的ODNP仪器尚未商业化。本章的目的是分享成功利用和推广ODNP技术所需的硬件、理论、测量和数据分析方法的技术诀窍。
