Rimoldi Guillermo, Uzal Francisco, Chin R P, Palombo Enzo A, Awad Milena, Lyras Dena, Shivaprasad H L
Avian Dis. 2015 Sep;59(3):447-51. doi: 10.1637/11077-033115-Case.1.
Three outbreaks of necrotic enteritis-like disease associated with Clostridium sordelii were diagnosed in commercial broiler chicken flocks with 18,000 to 31,000 birds between 18 and 26 days old. Clinical signs in the affected flocks included high mortality up to 2% a day, depression, and diarrhea. The main gross changes included segmental dilation of the small intestine with watery contents, gas, mucoid exudate, and roughened and uneven mucosa, occasionally covered with a pseudomembrane. Microscopic lesions in the small intestine were characterized by extensive areas of coagulative necrosis of the villi, fibrinous exudate in the lumen, and high numbers of large, Gram-positive rods, occasionally containing subterminal spores, seen in the necrotic tissue and lumen. These rods were identified as C. sordellii by immunohistochemistry. Clostridium sordellii was isolated in an almost pure culture from the intestine of affected birds. A retrospective study of commercial broiler chicken and turkey submissions to the California Animal Health and Food Safety Laboratory System revealed that C. sordellii had been isolated from intestinal lesions in outbreaks of necrotic enteritis-like disease in 8 of 39 cases, 5 times together with Clostridium perfringens and 3 times alone. The latter three cases are reported here.
在18至26日龄、存栏量为18000至31000只的商品肉鸡群中，诊断出三起与索氏梭菌相关的坏死性肠炎样疾病疫情。受影响鸡群的临床症状包括每天高达2%的高死亡率、精神沉郁和腹泻。主要的大体病变包括小肠节段性扩张，内容物呈水样、有气体、有黏液样渗出物，黏膜粗糙且不平整，偶尔覆盖有假膜。小肠的微观病变特征为绒毛广泛出现凝固性坏死区域、肠腔内有纤维蛋白渗出物，以及在坏死组织和肠腔内可见大量大型革兰氏阳性杆菌，偶尔含有亚末端孢子。通过免疫组织化学鉴定这些杆菌为索氏梭菌。从患病鸡的肠道中分离出几乎纯培养的索氏梭菌。对提交给加利福尼亚动物健康与食品安全实验室系统的商品肉鸡和火鸡样本进行的回顾性研究表明，在39例坏死性肠炎样疾病疫情的肠道病变中，有8例分离出了索氏梭菌，其中5次与产气荚膜梭菌同时分离出，3次单独分离出。后三例在此报告。
