Zhang Ershuai, Zhu Chuanshun, Yang Jun, Sun Hong, Zhang Xiaomin, Li Suhua, Wang Yonglan, Sun Lu, Yao Fanglian
School of Chemical Engineering and Technology, Key Laboratory of Systems Bioengineering of Ministry of Education, Tianjin University, Tianjin 300072, China.
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
Mater Sci Eng C Mater Biol Appl. 2016 Jan 1;58:278-85. doi: 10.1016/j.msec.2015.08.032. Epub 2015 Aug 28.
With the aim to explore a membrane system with appropriate degradation rate and excellent cell-occlusiveness for guided tissue regeneration (GTR), a series of poly(D, L-lactic acid) (PDLLA)/poly(D, L-lactic-co-glycolic acid) (PLGA) (100/0, 70/30, 50/50, 30/70, 0/100, w/w) composite membranes were fabricated via electrospinning. The fabricated membranes were evaluated by morphological characterization, water contact angle measurement and tensile test. In vitro degradation was characterized in terms of the weight loss and the morphological change. Moreover, in vitro cytologic research revealed that PDLLA/PLGA composite membranes could efficiently inhibit the infiltration of 293 T cells. Finally, subcutaneous implant test on SD rat in vivo showed that PDLLA/PLGA (70/30, 50/50) composite membranes could function well as a physical barrier to prevent cellular infiltration within 13 weeks. These results suggested that electrospun PDLLA/PLGA (50/50) composite membranes could serve as a promising barrier membrane for guided tissue regeneration due to suitable biodegradability, preferable mechanical properties and excellent cellular shielding effects.
为了探索一种具有合适降解速率和优异细胞封闭性的膜系统用于引导组织再生（GTR），通过静电纺丝制备了一系列聚（D，L-乳酸）（PDLLA）/聚（D，L-乳酸-共-乙醇酸）（PLGA）（100/0、70/30、50/50、30/70、0/100，重量比）复合膜。通过形态表征、水接触角测量和拉伸试验对制备的膜进行评估。体外降解通过重量损失和形态变化进行表征。此外，体外细胞学研究表明，PDLLA/PLGA复合膜可以有效抑制293 T细胞的浸润。最后，对SD大鼠进行体内皮下植入试验表明，PDLLA/PLGA（70/30、50/50）复合膜在13周内可以很好地作为物理屏障防止细胞浸润。这些结果表明，静电纺丝的PDLLA/PLGA（50/50）复合膜由于具有合适的生物降解性、较好的力学性能和优异的细胞屏蔽作用，有望成为引导组织再生的屏障膜。
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