Nayak Vasudev Vivekanand, Mirsky Nicholas Alexander, Slavin Blaire V, Witek Lukasz, Coelho Paulo G, Tovar Nick
Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
Biomaterials Division, College of Dentistry, New York University, New York, NY 10010, USA.
Materials (Basel). 2023 Oct 10;16(20):6633. doi: 10.3390/ma16206633.
Non-resorbable dental barrier membranes entail the risk of dehiscence due to their smooth and functionally inert surfaces. Non-thermal plasma (NTP) treatment has been shown to increase the hydrophilicity of a biomaterials and could thereby enhance cellular adhesion. This study aimed to elucidate the role of allyl alcohol NTP treatment of poly(tetrafluoroethylene) in its cellular adhesion. The materials (non-treated PTFE membranes (NTMem) and NTP-treated PTFE membranes (PTMem)) were subjected to characterization using scanning electron microscopy (SEM), contact angle measurements, X-ray photoelectron spectroscopy (XPS), and electron spectroscopy for chemical analysis (ESCA). Cells were seeded upon the different membranes, and cellular adhesion was analyzed qualitatively and quantitatively using fluorescence labeling and a hemocytometer, respectively. PTMem exhibited higher surface energies and the incorporation of reactive functional groups. NTP altered the surface topography and chemistry of PTFE membranes, as seen through SEM, XPS and ESCA, with partial defluorination and polymer chain breakage. Fluorescence labeling indicated significantly higher cell populations on PTMem relative to its untreated counterparts (NTMem). The results of this study support the potential applicability of allyl alcohol NTP treatment for polymeric biomaterials such as PTFE-to increase cellular adhesion for use as dental barrier membranes.
不可吸收性牙科屏障膜因其光滑且功能惰性的表面而存在裂开的风险。非热等离子体（NTP）处理已被证明可增加生物材料的亲水性，从而增强细胞黏附。本研究旨在阐明烯丙醇NTP处理聚四氟乙烯在其细胞黏附方面的作用。使用扫描电子显微镜（SEM）、接触角测量、X射线光电子能谱（XPS）和化学分析电子能谱（ESCA）对材料（未处理的聚四氟乙烯膜（NTMem）和NTP处理的聚四氟乙烯膜（PTMem））进行表征。将细胞接种在不同的膜上，分别使用荧光标记和血细胞计数器对细胞黏附进行定性和定量分析。PTMem表现出更高的表面能和反应性官能团的掺入。通过SEM、XPS和ESCA可以看出，NTP改变了聚四氟乙烯膜的表面形貌和化学性质，出现了部分脱氟和聚合物链断裂。荧光标记表明，相对于未处理的对应物（NTMem），PTMem上的细胞数量显著更多。本研究结果支持烯丙醇NTP处理在聚四氟乙烯等聚合物生物材料上的潜在适用性——增加细胞黏附以用作牙科屏障膜。
