Key Laboratory of Textile Science & Technology, Ministry of Education, Donghua University, Shanghai 201620, P. R. China.
J Biomater Sci Polym Ed. 2012;23(1-4):299-313. doi: 10.1163/092050610X550340. Epub 2011 Jan 18.
Organic/inorganic hybrid nanofiber systems have generated great interest in the area of tissue engineering and drug delivery. In this study, halloysite nanotube (HNT)-doped poly(lactic-co-glycolic acid) (PLGA) composite nanofibers were fabricated via electrospinning and the influence of the incorporation of HNTs within PLGA nanofibers on their in vitro biocompatibility was investigated. The morphology, mechanical and thermal properties of the composite nanofibers were characterized by scanning electron microscopy (SEM), tensile test, differential scanning calorimetry and thermogravimetric analysis. The adhesion and proliferation of mouse fibroblast cells cultured on both PLGA and HNT-doped PLGA fibrous scaffolds were compared through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay of cell viability and SEM observation of cell morphology. We show that the morphology of the PLGA nanofibers does not appreciably change with the incorporation of HNTs, except that the mean diameter of the fibers increased with the increase of HNT incorporation in the composite. More importantly, the mechanical properties of the nanofibers were greatly improved. Similar to electrospun PLGA nanofibers, HNT-doped PLGA nanofibers were able to promote cell attachment and proliferation, suggesting that the incorporation of HNTs within PLGA nanofibers does not compromise the biocompatibility of the PLGA nanofibers. In addition, we show that HNT-doped PLGA scaffolds allow more protein adsorption than those without HNTs, which may provide sufficient nutrition for cell growth and proliferation. The developed electrospun HNT-doped composite fibrous scaffold may find applications in tissue engineering and pharmaceutical sciences.
有机/无机杂化纳米纤维系统在组织工程和药物输送领域引起了极大的兴趣。在这项研究中，通过静电纺丝制备了多壁纳米管（HNT）掺杂的聚（乳酸-共-羟基乙酸）（PLGA）复合纳米纤维，并研究了 HNTs 在 PLGA 纳米纤维中的掺入对其体外生物相容性的影响。通过扫描电子显微镜（SEM）、拉伸试验、差示扫描量热法和热重分析对复合纳米纤维的形貌、力学和热性能进行了表征。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）法测定细胞活力和 SEM 观察细胞形态，比较了培养在 PLGA 和 HNT 掺杂的 PLGA 纤维支架上的小鼠成纤维细胞的黏附和增殖情况。结果表明，除纤维的平均直径随复合纤维中 HNT 含量的增加而增加外，PLGA 纳米纤维的形态没有明显变化。更重要的是，纳米纤维的力学性能得到了很大的提高。与静电纺丝的 PLGA 纳米纤维类似，HNT 掺杂的 PLGA 纳米纤维能够促进细胞黏附和增殖，表明 HNTs 在 PLGA 纳米纤维中的掺入不会影响 PLGA 纳米纤维的生物相容性。此外，我们还表明，HNT 掺杂的 PLGA 支架比不含 HNTs 的支架具有更好的蛋白质吸附能力，这可能为细胞生长和增殖提供足够的营养。开发的静电纺丝 HNT 掺杂复合纤维支架可能在组织工程和药物科学领域有应用前景。
