关于脂质转移蛋白的作用。拟南芥ltp3突变体在萌发和幼苗生长方面存在缺陷。
On the role of a Lipid-Transfer Protein. Arabidopsis ltp3 mutant is compromised in germination and seedling growth.
作者信息
Pagnussat Luciana A, Oyarburo Natalia, Cimmino Carlos, Pinedo Marcela L, de la Canal Laura
机构信息
a Instituto de Investigaciones Biológicas; Universidad Nacional de Mar del Plata - CONICET ; Mar del Plata, Argentina.
b Centro de Biotecnología Advanta Semillas S.A.I.C. ; Balcarce, Argentina.
出版信息
Plant Signal Behav. 2015;10(12):e1105417. doi: 10.1080/15592324.2015.1105417.
Abstract
Plant Lipid-Transfer Proteins (LTPs) exhibit the ability to reversibly bind/transport lipids in vitro. LTPs have been involved in diverse physiological processes but conclusive evidence on their role has only been presented for a few members, none of them related to seed physiology. Arabidopsis seeds rely on storage oil breakdown to supply carbon skeletons and energy for seedling growth. Here, Arabidopsis ltp3 mutant was analyzed for its ability to germinate and for seedling establishment. Ltp3 showed delayed germination and reduced germination frequency. Seedling growth appeared reduced in the mutant but this growth restriction was rescued by the addition of an exogenous carbon supply, suggesting a defective oil mobilization. Lipid breakdown analysis during seedling growth revealed a differential profile in the mutant compared to the wild type. The involvement of LTP3 in germination and seedling growth and its relationship with the lipid transfer ability of this protein is discussed.
摘要
植物脂质转运蛋白（LTPs）在体外具有可逆结合/转运脂质的能力。LTPs参与了多种生理过程，但仅对少数成员有关于其作用的确凿证据，且这些成员均与种子生理学无关。拟南芥种子依靠储存油的分解来为幼苗生长提供碳骨架和能量。在此，对拟南芥ltp3突变体的发芽能力和幼苗建立情况进行了分析。Ltp3表现出发芽延迟和发芽频率降低。突变体中的幼苗生长似乎受到抑制，但通过添加外源碳源可挽救这种生长受限的情况，这表明存在油动员缺陷。幼苗生长期间的脂质分解分析显示，与野生型相比，突变体具有不同的特征。本文讨论了LTP3在发芽和幼苗生长中的作用及其与该蛋白质脂质转运能力的关系。
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