大肠杆菌细菌铁蛋白的分子大小与对称性。四种晶体形式的X射线晶体学表征

Molecular size and symmetry of the bacterioferritin of Escherichia coli. X-ray crystallographic characterization of four crystal forms.

作者信息

Smith J M, Ford G C, Harrison P M, Yariv J, Kalb A J

机构信息

Department of Biochemistry, University of Sheffield, England.

出版信息

J Mol Biol. 1989 Jan 20;205(2):465-7. doi: 10.1016/0022-2836(89)90358-6.

DOI:10.1016/0022-2836(89)90358-6

PMID:2648005

Abstract

X-ray crystallographic data from four crystal forms of Escherichia coli bacterioferritin show that the molecule has a diameter in the range 119 to 128 A. Molecules are composed of 24 subunits arranged in 432 symmetry. In both size and symmetry the molecule resembles ferritin from eukaryotes. The four crystal forms are monoclinic, space group P2(1) with unit cell dimensions a = 118.7 A, b = 211.6 A, c = 123.3 A and beta = 119.1 degrees; orthorhombic, C222(1), a = 128.7 A, b = 197.1 A, c = 202.8 A; tetragonal, P4(2)2(1)2, a = b = 210.6 A, c = 145.0 A and cubic, I432, a = 146.9 A.

摘要

来自大肠杆菌细菌铁蛋白四种晶体形式的X射线晶体学数据表明，该分子的直径在119至128埃范围内。分子由以432对称排列的24个亚基组成。在大小和对称性方面，该分子类似于真核生物的铁蛋白。这四种晶体形式分别是单斜晶系，空间群P2(1)，晶胞参数a = 118.7埃，b = 211.6埃，c = 123.3埃，β = 119.1度；正交晶系，C222(1)，a = 128.7埃，b = 197.1埃，c = 202.8埃；四方晶系，P4(2)2(1)2，a = b = 210.6埃，c = 145.0埃；以及立方晶系，I432，a = 146.9埃。