van Eerde André, Wolterink-van Loo Suzanne, van der Oost John, Dijkstra Bauke W
Laboratory of Biophysical Chemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt 11):1061-6. doi: 10.1107/S1744309106039583. Epub 2006 Oct 25.
Escherichia coli bacterioferritin was serendipitously crystallized in a novel cubic crystal form and its structure could be determined to 2.5 A resolution despite a high degree of merohedral twinning. This is the first report of crystallographic data on 'as-isolated' E. coli bacterioferritin. The ferroxidase active site contains positive difference density consistent with two metal ions that had co-purified with the protein. X-ray fluorescence studies suggest that the metal composition is different from that of previous structures and is a mix of zinc and native iron ions. The ferroxidase-centre configuration displays a similar flexibility as previously noted for other bacterioferritins.
大肠杆菌细菌铁蛋白意外地以一种新型立方晶型结晶，尽管存在高度的merohedral孪晶，但仍能将其结构解析到2.5埃的分辨率。这是关于“原样分离”的大肠杆菌细菌铁蛋白晶体学数据的首次报告。铁氧化酶活性位点含有与两个与蛋白质共纯化的金属离子一致的正差密度。X射线荧光研究表明，金属组成与先前结构不同，是锌和天然铁离子的混合物。铁氧化酶中心构型显示出与先前其他细菌铁蛋白所指出的类似的灵活性。
