Smith J M, Quirk A V, Plank R W, Diffin F M, Ford G C, Harrison P M
Department of Biochemistry, University of Sheffield, U.K.
Biochem J. 1988 Oct 15;255(2):737-40.
Two different procedures were used to prepare pure samples of 'cytochrome b1' (column chromatography) and 'bacterioferritin' (immunoprecipitation) from Escherichia coli K-12 strain CA265. Both were crystallized, and X-ray-crystallographic data were compared with those from the bacterioferritin of E. coli strain W3300 used as a standard. We conclude that 'cytochrome b1' and 'bacterioferritin' are identical.
采用两种不同的方法从大肠杆菌K-12菌株CA265中制备“细胞色素b1”(柱色谱法)和“细菌铁蛋白”(免疫沉淀法)的纯样品。二者均已结晶,并将X射线晶体学数据与用作标准的大肠杆菌W3300菌株的细菌铁蛋白的数据进行了比较。我们得出结论,“细胞色素b1”和“细菌铁蛋白”是相同的。