Baer M R, Gao X Z, Sato H, Singh P, Preisler H D
Department of Hematologic Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
Leuk Res. 1989;13(2):165-72. doi: 10.1016/0145-2126(89)90141-0.
Peripheral blood mononuclear cells cultured in liquid medium with fetal calf serum undergo differentiation to myeloblasts (7 days) and then mature granulocytes (14-21 days). This culture system was used to study proto-oncogene expression during pre-myeloblast myeloid differentiation. c-myc mRNA was present in the peripheral blood mononuclear cells placed into culture, fell during the first 2-4 days of culture and then rose between days 2-4 and day 7 of culture, prior to and coincident with the appearance of myeloblasts. Histone H3 mRNA was absent or present at very low levels at initiation of cultures, and then rose throughout the first 7 days of culture. c-fms mRNA was absent at initiation of cultures, and appeared on days 2-5 of culture, prior to the appearance of myeloblasts. c-fos mRNA was not detected during differentiation of peripheral blood mononuclear cells to myeloblasts. Elucidation of patterns of proto-oncogene expression during normal myeloid differentiation is a prerequisite for interpretation of proto-oncogene expression in myeloid leukemia cells.
在含有胎牛血清的液体培养基中培养的外周血单个核细胞会分化为成髓细胞（7天），然后再分化为成熟粒细胞（14 - 21天）。该培养系统用于研究原癌基因在成髓细胞前髓系分化过程中的表达。c-myc mRNA在放入培养的外周血单个核细胞中存在，在培养的前2 - 4天下降，然后在培养的第2 - 4天至第7天之间上升，先于并与成髓细胞的出现同时发生。组蛋白H3 mRNA在培养开始时不存在或水平极低，然后在培养的前7天内持续上升。c-fms mRNA在培养开始时不存在，在培养的第2 - 5天出现，先于成髓细胞的出现。在外周血单个核细胞分化为成髓细胞的过程中未检测到c-fos mRNA。阐明正常髓系分化过程中原癌基因的表达模式是解释髓系白血病细胞中原癌基因表达的先决条件。
