Liu Guo-Xia, Ma Hong-Mei, Xie Hong-Yan, Xuan Ning, Picimbon Jean-François
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Jinan, PR China.
Gene. 2016 Jan 15;576(1 Pt 2):284-91. doi: 10.1016/j.gene.2015.10.036. Epub 2015 Oct 19.
Bemisia tabaci Gennadius biotypes B and Q are two of the most important worldwide agricultural insect pests. Genomic sequences of Type-2 B. tabaci chemosensory protein (BtabCSP2) were cloned and sequenced in B and Q biotypes, revealing key biotype-specific variations in the intron sequence. A Q260 sequence was found specifically in Q-BtabCSP2 and Cucumis melo LN692399, suggesting ancestral horizontal transfer of gene between the insect and the plant through bacteria. A cleaved amplified polymorphic sequences (CAPS) method was then developed to differentiate B and Q based on the sequence variation in exon of BtabCSP2 gene. The performances of CSP2-based CAPS for whitefly recognition were assessed using B. tabaci field collections from Shandong Province (P.R. China). Our SacII based CAPS method led to the same result compared to mitochondrial cytochrome oxidase-based CAPS method in the field collections. We therefore propose an explanation for CSP origin and a new rapid simple molecular method based on genomic DNA and chemosensory gene to differentiate accurately the B and Q whiteflies of the Bemisia complex around the world.
烟粉虱Bemisia tabaci Gennadius生物型B和Q是全球最重要的两种农业害虫。对2型烟粉虱化学感受蛋白（BtabCSP2）的基因组序列在生物型B和Q中进行了克隆和测序，揭示了内含子序列中关键的生物型特异性变异。在Q-BtabCSP2和甜瓜Cucumis melo LN692399中特异性发现了一个Q260序列，表明该基因通过细菌在昆虫和植物之间发生了祖先水平转移。随后开发了一种酶切扩增多态性序列（CAPS）方法，基于BtabCSP2基因外显子的序列变异来区分生物型B和Q。使用来自中国山东省的烟粉虱田间样本评估了基于CSP2的CAPS对粉虱识别的性能。在田间样本中，我们基于SacII的CAPS方法与基于线粒体细胞色素氧化酶的CAPS方法得出了相同的结果。因此，我们对CSP的起源提出了一种解释，并提出了一种基于基因组DNA和化学感受基因的新的快速简单分子方法，以准确区分世界各地烟粉虱复合体中的生物型B和Q粉虱。
