Beppe Galba Jean, Dongmo Alain Bertrand, Foyet Harquin Simplice, Dimo Théophile, Mihasan Marius, Hritcu Lucian
Laboratory of Animal Physiology, Faculty of Science, University of Yaoundé I, PO Box, 812, Yaoundé, Cameroon.
Department of Biological Sciences, Faculty of Science, University of Maroua, PO Box, 814, Maroua, Cameroon.
BMC Complement Altern Med. 2015 Oct 19;15:374. doi: 10.1186/s12906-015-0912-0.
While the Albizia adianthifolia (Schumach.) W. Wright (Fabaceae) is a traditional herb largely used in the African traditional medicine as analgesic, purgative, antiinflammatory, antioxidant, antimicrobial, memory-enhancer, anxiolytic and antidepressant drug, there are no scientific data that clarify the anxiolytic and antidepressant-like effects in 6-hydroxydopamine (6-OHDA)-lesioned animal model of Parkinson's disease. This study was undertaken in order to identify the effects of aqueous extract of A. adianthifolia leaves on 6-hydroxydopamine-induced anxiety, depression and oxidative stress in the rat amygdala.
The effect of the aqueous extract of A. adianthifolia leaves (150 and 300 mg/kg, orally, daily, for 21 days) on anxiety and depression was assessed using elevated plus-maze and forced swimming tests, as animal models of anxiety and depression. Also, the antioxidant activity in the rat amygdala was assessed using assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels. Statistical analyses were performed using by one-way analysis of variance (ANOVA). Significant differences were determined by Tukey's post hoc test. F values for which p < 0.05 were regarded as statistically significant. Pearson's correlation coefficient and regression analysis were used in order to evaluate the connection between behavioral measures, the antioxidant defence and lipid peroxidation.
6-OHDA-lesioned rats exhibited the following: decrease of the exploratory activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming time and increase of immobility time within forced swimming test. Administration of the aqueous extract significantly exhibited anxiolytic- and antidepressant-like effects and also antioxidant potential in the rat amygdala.
Our results suggest that the aqueous extract ameliorates 6-OHDA-induced anxiety and depression by attenuation of the oxidative stress in the rat amygdala. These pieces of evidence accentuate its use in traditional medicine.
虽然合欢树（豆科）是一种传统草药，在非洲传统医学中被广泛用作镇痛药、泻药、抗炎药、抗氧化剂、抗菌剂、记忆增强剂、抗焦虑药和抗抑郁药，但尚无科学数据阐明其在帕金森病6-羟基多巴胺（6-OHDA）损伤动物模型中的抗焦虑和抗抑郁样作用。本研究旨在确定合欢树叶水提取物对6-羟基多巴胺诱导的大鼠杏仁核焦虑、抑郁和氧化应激的影响。
采用高架十字迷宫和强迫游泳试验评估合欢树叶水提取物（150和300 mg/kg，口服，每日，共21天）对焦虑和抑郁的影响，作为焦虑和抑郁的动物模型。此外，通过超氧化物歧化酶、谷胱甘肽过氧化物酶和过氧化氢酶的比活性、还原型谷胱甘肽的总含量、蛋白质羰基和丙二醛水平评估大鼠杏仁核中的抗氧化活性。采用单因素方差分析（ANOVA）进行统计分析。通过Tukey事后检验确定显著差异。p < 0.05的F值被视为具有统计学意义。使用Pearson相关系数和回归分析来评估行为指标、抗氧化防御和脂质过氧化之间的联系。
6-OHDA损伤的大鼠表现出以下情况：在高架十字迷宫试验中探索活动减少、在开放臂中花费的时间百分比和进入次数减少，以及在强迫游泳试验中游泳时间减少和不动时间增加。给予水提取物在大鼠杏仁核中显著表现出抗焦虑和抗抑郁样作用以及抗氧化潜力。
我们的结果表明，水提取物通过减轻大鼠杏仁核中的氧化应激来改善6-OHDA诱导的焦虑和抑郁。这些证据突出了其在传统医学中的应用。
Zotero 插件
