Wang Chuanyong, Zhao Yongqi, Zheng Sainan, Xue Jing, Zhou Jinglin, Tang Yi, Jiang Li, Li Wei
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Department of Stomatology, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Scanning. 2015 Sep-Oct;37(5):313-21. doi: 10.1002/sca.21218. Epub 2015 Apr 24.
We explore the influence of enamel surface morphology on nanoscale bacterial adhesion forces. Three dimensional morphology characteristics of enamel slices, which were treated with phosphoric acid (for 0 s, 5 s, 10 s, 20 s, and 30 s), were acquired. Adhesion forces of three initial colonizers (Streptococcus oralis, Streptococcus sanguinis, and Streptococcus mitis) and two cariogenic bacterial strains (Streptococcus mutans and Streptococcus sobrinus) with etched enamel surfaces were determined. Comparison of the forces was made by using bacterial probe method under atomic force microscope (AFM) in adhesion buffer. The results showed that enamel morphology was significantly altered by etching treatment. The roughness, peak-to-valley height, and valley-to-valley width of the depth profile, surface area, and volume increased linearly with acid exposure time, and reached the maximum at 30s, respectively. The adhesion forces of different strains increased accordingly with etching time. Adhesion forces of S. oralis, S. mitis, S. mutans, and S. sobrinus reached the maximum values of 0.81 nN, 0.84 nN, 0.73 nN, and 0.64 nN with enamel treated for 20s, respectively, whereas that of S. sanguinis at 10s (1.28nN), and dropped on coarser enamel surfaces. In conclusion, enamel micro-scale morphology may significantly alter the direct adhesion forces of bacteria. And there may be a threshold roughness for bacterial adhesion on enamel surface.
我们探究了牙釉质表面形态对纳米级细菌粘附力的影响。获取了经磷酸处理（处理时间分别为0秒、5秒、10秒、20秒和30秒）的牙釉质切片的三维形态特征。测定了三种早期定植菌（口腔链球菌、血链球菌和缓症链球菌）以及两种致龋菌株（变形链球菌和远缘链球菌）在蚀刻牙釉质表面的粘附力。在原子力显微镜（AFM）下，于粘附缓冲液中采用细菌探针法对这些力进行了比较。结果表明，蚀刻处理显著改变了牙釉质形态。深度轮廓的粗糙度、峰谷高度和谷谷宽度、表面积以及体积均随酸暴露时间呈线性增加，并分别在30秒时达到最大值。不同菌株的粘附力随蚀刻时间相应增加。口腔链球菌、缓症链球菌、变形链球菌和远缘链球菌在牙釉质处理20秒时的粘附力分别达到最大值0.81 nN、0.84 nN、0.73 nN和0.64 nN，而血链球菌在10秒时达到最大值（1.28 nN），在更粗糙的牙釉质表面则下降。总之，牙釉质微观形态可能显著改变细菌的直接粘附力。并且牙釉质表面细菌粘附可能存在一个粗糙度阈值。
