Experimental Physics and Center for Biophysics, Saarland University, 66123 Saarbrücken, Germany.
Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University, 66421 Homburg/Saar, Germany.
ACS Biomater Sci Eng. 2022 Apr 11;8(4):1476-1485. doi: 10.1021/acsbiomaterials.1c00925. Epub 2022 Mar 9.
Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For medical application of materials, particular attention has to be paid to biocompatibility, osseointegration, and bacterial adhesion behavior. To understand their properties and behavior, experimental studies with natural materials such as teeth are strongly required. The results, however, may be highly case-dependent because natural surfaces have the disadvantage of being subject to wide variations, for instance in their chemical composition, structure, morphology, roughness, and porosity. A synthetic surface which mimics enamel in its performance with respect to bacterial adhesion and biocompatibility would, therefore, facilitate systematic studies much better. In this study, we discuss the possibility of using hydroxyapatite (HAp) pellets to simulate the surfaces of teeth and show the possibility and limitations of using a model surface. We performed single-cell force spectroscopy with single cells to measure adhesion-related parameters such as adhesion force and rupture length of cell wall proteins binding to HAp and enamel. We also examine the influence of blood plasma and saliva on the adhesion properties of . The results of these measurements are matched to water wettability, elemental composition of the samples, and the change in the macromolecules adsorbed over time on the surface. We found that the adhesion properties of were similar on HAp and enamel samples under all conditions: Significant decreases in adhesion strength were found equally in the presence of saliva or blood plasma on both surfaces. We therefore conclude that HAp pellets are a good alternative for natural dental material. This is especially true when slight variations in the physicochemical properties of the natural materials may affect the experimental series.
研究人员从天然或合成表面中汲取灵感，以开发用于医学应用的材料，这与许多其他研究方向类似。对于医用材料，尤其需要关注生物相容性、骨整合和细菌黏附行为。为了了解其性能和行为，强烈需要对牙齿等天然材料进行实验研究。然而，结果可能高度依赖于具体情况，因为天然表面存在很大的变异性，例如在其化学成分、结构、形态、粗糙度和孔隙率方面。因此，一种在细菌黏附和生物相容性方面模仿牙釉质性能的合成表面将更有利于系统研究。在这项研究中，我们讨论了使用羟基磷灰石（HAp）颗粒模拟牙齿表面的可能性，并展示了使用模型表面的可能性和局限性。我们使用单细胞力谱法对单个细胞进行了测量，以测量与黏附相关的参数，如黏附力和细胞壁蛋白与 HAp 和牙釉质结合的断裂长度。我们还研究了血液和唾液对黏附性能的影响。这些测量结果与样品的润湿性、元素组成以及随时间在表面上吸附的大分子的变化相匹配。结果表明，在所有条件下，细菌在 HAp 和牙釉质样品上的黏附特性相似：在两种表面上，唾液或血液的存在都会导致黏附强度明显下降。因此，我们得出结论，HAp 颗粒是天然牙科材料的良好替代品。当天然材料的物理化学性质的微小变化可能影响实验系列时，尤其如此。
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