Sainan Zheng, Li Jiang, Lei Zhang, Liying Hao, Lu Ye, Wei Li
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2016 Oct 1;34(5):448-453. doi: 10.7518/hxkq.2016.05.003.
This study is to determine the common oral streptococcal adhesion forces by using composite resin and glass ionomer cement (GIC) with different degrees of surface roughness via atomic force microscopy (AFM) analysis. The influence of surface roughness on bacterial adhesion force is also discussed.
Polishing and grinding were applied to obtain 300, 200, 100, and 10 nm surfaces of light-cured composite resin and GIC samples. Surface topography was assessed by AFM analysis. Initial colonizers (Streptococcus sanguinis and Streptococcus mitis) and cariogenic bacterial strains (Streptococcus mutans and Streptococcus sobrinus) were used to obtain bacteria-modified AFM probes. The force-distance curves were also measured by AFM analysis to determine the adhesion forces of bacteria on the surfaces of the composite resin and GIC. Material surface roughness was analyzed using ANOVA, and adhesion forces were subjected to nonparametric analysis (Kruskal-Wallis test). Comparison among groups was performed by Dunn's test. Material surface roughness and bacterial adhesion forces were subjected to correlation analysis.
Bacterial adhesion forces increased with increasing material roughness. The adhesion forces of the four bacterial species reached the maximum on the material surface of 300 nm. The adhesion force of Streptococcus mutans increased from 0.578 nN to 2.876 nN on GIC surfaces with 10 and 300 nm roughness. The adhesion forces of the four species on the surface of the composite resin were stronger than that of GIC. The initial colonizers exhibited stronger adhesion forces to different materials than the cariogenic strains. Intergroup differences were evident on the 200 and 300 nm material surfaces.
The surface roughness of the material significantly affected the bacterial adhesion forces, and a significant linear correlation existed between both factors. The bacterial adhesion forces of the GIC were lower than that of the composite resin. Furthermore, surface roughness exhibited less influence on the cariogenic strains than that on the initial colonizers.
本研究旨在通过原子力显微镜（AFM）分析，测定不同表面粗糙度的复合树脂和玻璃离子水门汀（GIC）上常见口腔链球菌的黏附力。同时探讨表面粗糙度对细菌黏附力的影响。
对光固化复合树脂和GIC样本进行抛光和研磨，以获得300、200、100和10 nm的表面。通过AFM分析评估表面形貌。使用早期定植菌（血链球菌和缓症链球菌）和致龋菌株（变形链球菌和远缘链球菌）制备细菌修饰的AFM探针。还通过AFM分析测量力-距离曲线，以确定细菌在复合树脂和GIC表面的黏附力。使用方差分析（ANOVA）分析材料表面粗糙度，对黏附力进行非参数分析（Kruskal-Wallis检验）。通过Dunn检验进行组间比较。对材料表面粗糙度和细菌黏附力进行相关性分析。
细菌黏附力随材料粗糙度的增加而增加。四种细菌在300 nm的材料表面上黏附力达到最大值。在粗糙度为10和300 nm的GIC表面上，变形链球菌的黏附力从0.578 nN增加到2.876 nN。四种细菌在复合树脂表面的黏附力比在GIC表面更强。早期定植菌对不同材料的黏附力比致龋菌株更强。在200和300 nm的材料表面上，组间差异明显。
材料的表面粗糙度显著影响细菌黏附力，且这两个因素之间存在显著的线性相关性。GIC的细菌黏附力低于复合树脂。此外，表面粗糙度对致龋菌株的影响小于对早期定植菌的影响。
