乐果对雄性小鼠生殖参数的影响。
Effects of dimethoate in male mice reproductive parameters.
作者信息
Jallouli Manel, Dhouib Ines El Bini, Dhouib Hanène, Gharbi Najoua, El Fazaa Saloua
机构信息
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
Laboratory of Aggression Physiology and Endocrine Metabolic Studies, Department of Biology, Faculty of Sciences, El Manar University Tunis, 2092, Tunisia.
出版信息
Regul Toxicol Pharmacol. 2015 Dec;73(3):853-8. doi: 10.1016/j.yrtph.2015.10.010. Epub 2015 Oct 19.
The aim of the current study was to investigate the ability of dimethoate (DMT) to induce reprotoxicity in male mice. The dose (20 mg/kg/day) was given orally for 30 days. A significant decrease in sperm count, motility and viability and a significant increase of morphologically abnormal spermatozoa percent in DMT treated mice was observed. Testicular Acetylcholinesterase (AChE) and Butyrylcholinesterase (BChE) activities were inhibited. Also, a significant increase in lipid peroxidation level and a significant decrease in the activities of antioxidant enzymes were observed in testis of DMT mice. In addition, gene expression of glutathione peroxidase 4 (GPx4) was quantified in RNA samples extracted from the testis by real-time reverse transcription-polymerase chain reaction (RT-PCR). Compared with control, mRNA expression of GPx4 was slightly decreased after DMT-exposure.
本研究的目的是调查乐果（DMT）诱导雄性小鼠生殖毒性的能力。以20毫克/千克/天的剂量口服给药30天。观察到乐果处理的小鼠精子数量、活力和存活率显著下降，形态异常精子百分比显著增加。睾丸乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）活性受到抑制。此外，在乐果处理的小鼠睾丸中观察到脂质过氧化水平显著升高，抗氧化酶活性显著降低。另外，通过实时逆转录-聚合酶链反应（RT-PCR）对从睾丸中提取的RNA样本中谷胱甘肽过氧化物酶4（GPx4）的基因表达进行了定量。与对照组相比，乐果暴露后GPx4的mRNA表达略有下降。
Zotero 插件