位于印缅生物多样性热点地区的穆伦国家公园的细菌多样性：一种宏基因组学方法。
Bacterial diversity of Murlen National Park located in Indo-Burman Biodiversity hotspot region: A metagenomic approach.
作者信息
De Mandal Surajit, Lalremsanga H T, Kumar Nachimuthu Senthil
机构信息
Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.
Department of Zoology, Mizoram University, Aizawl 796004, Mizoram, India.
出版信息
Genom Data. 2015 May 8;5:25-6. doi: 10.1016/j.gdata.2015.04.025. eCollection 2015 Sep.
Paired end Illumina Mi-Seq sequencing of 16S rRNA gene amplicon was carried out to study the bacterial community in the soil of Murlen National Park located in Indo-Burman Biodiversity hotspot region. Metagenome consisted of 302,416 reads with 151.81 Mb data and G + C content of 56.48%. More than 85% sequence was having a Phred score >=Q30 and individual sequence length was 251 bp. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. SRP057136. Community metagenomics revealed a total of 1802 species belonging to 29 different phyla dominated by Acidobacteria (39.45%), Proteobacteria (26.95%) and Planctomycetes (7.81%). Our data detected a wide group of bacterial community which will be useful in further isolating and characterizing the economic importance of bacteria from this region.
对16S rRNA基因扩增子进行配对末端Illumina Mi-Seq测序，以研究位于印度-缅甸生物多样性热点地区的穆尔伦国家公园土壤中的细菌群落。宏基因组由302416条 reads组成，数据量为151.81 Mb，G + C含量为56.48%。超过85%的序列的Phred质量分数≥Q30，单个序列长度为251 bp。宏基因组序列数据可在NCBI的生物项目数据库中获取，登录号为SRP057136。群落宏基因组学揭示了总共1802个物种，属于29个不同的门，以酸杆菌门（39.45%）、变形菌门（26.95%）和浮霉菌门（7.81%）为主。我们的数据检测到了广泛的细菌群落，这将有助于进一步分离和鉴定该地区具有经济重要性的细菌。
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