基于扩增子测序对北极克罗斯峡湾细菌多样性的分析。
Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic.
作者信息
Kachiprath Bhavya, Puthumana Jayesh, Gopi Jayanath, Solomon Solly, Krishnan K P, Philip Rosamma
机构信息
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi 682016, Kerala, India.
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi 682016, Kerala, India.
出版信息
Data Brief. 2018 Nov 27;21:2522-2525. doi: 10.1016/j.dib.2018.11.101. eCollection 2018 Dec.
In this study, Illumina Miseq sequencing of 16S rRNA gene amplicon was performed on sediments collected from Krossfjorden, Arctic for analyzing the bacterial community structure. Metagenome contained 15,936 sequences with 5,809,491 bp size and 53% G+C content. Metagenome sequence information are now available at NCBI under the Sequence Read Archive (SRA) database with accession no. SRP159159. Taxonomic hits distribution from MG-RAST analysis revealed the dominance of Alpha- and Gamma-subdivisions of Proteobacteria (88.89%) along with Bacteriodetes (8.89%) and Firmicutes (2.22%). Predominant species were TW-7 (24%), (20%) and spp. SM9913 (18%). MG-RAST assisted analysis also detected the presence of a variety of marine taxa like etc. which take part in key ecological functions and biogeochemical activities of Arctic fjord ecosystems.
在本研究中，对从北极克罗斯峡湾采集的沉积物进行了16S rRNA基因扩增子的Illumina Miseq测序，以分析细菌群落结构。宏基因组包含15,936个序列，大小为5,809,491 bp，G+C含量为53%。宏基因组序列信息现已在NCBI的序列读取存档（SRA）数据库中提供，登录号为SRP159159。MG-RAST分析的分类命中分布显示，变形菌门的α-和γ-亚类占主导地位（88.89%），同时还有拟杆菌门（8.89%）和厚壁菌门（2.22%）。优势物种为TW-7（24%）、（20%）和SM9913菌属（18%）。MG-RAST辅助分析还检测到多种海洋分类群的存在，如等，它们参与北极峡湾生态系统的关键生态功能和生物地球化学活动。
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