De Mandal Surajit, Panda Amrita Kumari, Lalnunmawii Esther, Bisht Satpal Singh, Kumar Nachimuthu Senthil
Department of Biotechnology, Mizoram University, Aizawl 796004, Mizoram, India.
Genom Data. 2015 May 8;5:13-4. doi: 10.1016/j.gdata.2015.04.023. eCollection 2015 Sep.
Bacterial community of the Khuangcherapuk cave sediment was assessed by Illumina amplicon sequencing. The metagenome comprised of 533,120 raw reads with an average base quality (Phred score) 36.75 and G + C content is 57.61%. A total of 18 bacterial phyla were detected with following abundant genus - Mycobacterium (21.72%), Rhodococcus (7.09%), Alteromonas (1.42%), Holomonas (0.7%) and Salinisphaera (0.20%). Majority portion of the sequences (68%) is unclassified at the genus level indicating the possibilities for the presence of novel species in this cave. This study reports the cave bacterial diversity from the biodiversity hotspot region of Eastern Himalayas. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. SRP056890.
通过Illumina扩增子测序评估了孔切拉普克洞穴沉积物的细菌群落。宏基因组由533,120条原始读数组成,平均碱基质量(Phred评分)为36.75,G + C含量为57.61%。共检测到18个细菌门,以下是丰度较高的属——分枝杆菌属(21.72%)、红球菌属(7.09%)、交替单胞菌属(1.42%)、全噬菌属(0.7%)和盐球形菌属(0.20%)。大部分序列(68%)在属水平上未分类,这表明该洞穴中可能存在新物种。本研究报告了东喜马拉雅生物多样性热点地区的洞穴细菌多样性。宏基因组序列数据可在NCBI的生物项目数据库中获取,登录号为SRP056890。