Kuenne Carsten, Guenther Stefan, Looso Mario, Zhang Ting, Krueger Marcus, Zhou Yonggang, Braun Thomas, Kim Johnny
Max-Planck-Institute for Heart and Lung Research, Department of Cardiac Development and Remodeling, Bad Nauheim, Germany.
Genom Data. 2015 Jun 3;5:122-5. doi: 10.1016/j.gdata.2015.05.013. eCollection 2015 Sep.
Satellite cells (SCs) represent a distinct population of stem cells, essential for maintenance, growth and regeneration of adult skeletal muscle. SCs are mononuclear and are located between the basal lamina and the plasma membrane of myofibers. They are typically characterized by presence of the transcription factor paired-box 7 (PAX7) that is widely used as a satellite cell marker. Under normal physiological conditions SCs are quiescent but are activated by insults such as injury, disease or exercise. Once activated, satellite cells proliferate and subsequently differentiate into myoblasts to finally fuse to form new myofibers or with preexisting myofibers to repair or rebuild the skeletal muscle. A minority of SCs retains stem cell characteristics and self-renews to assure future bouts of regeneration throughout most of adult life. While a comprehensive picture of the regulatory events controlling SC fate has not yet been achieved, several factors were recently identified playing important roles in functional processes. One example is the arginine methyltransferase Prmt5 that is known to have multiple roles in germ cells and is involved in the maintenance of ES cell pluripotency. We have previously shown that Prmt5 is required for muscle stem cell proliferation and regenerative myogenesis due to direct epigenetic regulation of the cell cycle inhibitor p21. Here we provide a dataset that investigates the loss of Prmt5 in isolated Pax7(+) primary SCs using the Pax7(CreERT2)/Prmt5(loxP/loxP) knockout mouse model. RNA-Seq raw and analyzed data have been deposited in GEO under accession code GSE66822.
卫星细胞（SCs）是一类独特的干细胞群体，对成年骨骼肌的维持、生长和再生至关重要。卫星细胞为单核细胞，位于肌纤维的基膜和质膜之间。它们的典型特征是存在转录因子配对盒7（PAX7），该因子被广泛用作卫星细胞标志物。在正常生理条件下，卫星细胞处于静止状态，但会因损伤、疾病或运动等刺激而被激活。一旦被激活，卫星细胞就会增殖，随后分化为成肌细胞，最终融合形成新的肌纤维，或与已有的肌纤维融合以修复或重建骨骼肌。少数卫星细胞保留干细胞特征并进行自我更新，以确保在成年后的大部分时间里能够进行后续的再生过程。虽然尚未全面了解控制卫星细胞命运的调控事件，但最近发现了几个在功能过程中发挥重要作用的因素。一个例子是精氨酸甲基转移酶Prmt5，已知它在生殖细胞中具有多种作用，并参与维持胚胎干细胞的多能性。我们之前已经表明，由于对细胞周期抑制剂p21的直接表观遗传调控，Prmt5是肌肉干细胞增殖和再生性肌生成所必需的。在这里，我们提供了一个数据集，该数据集使用Pax7（CreERT2）/Prmt5（loxP/loxP）基因敲除小鼠模型研究了分离的Pax7（+）原代卫星细胞中Prmt5的缺失情况。RNA-Seq原始数据和分析数据已存入基因表达综合数据库（GEO），登录号为GSE66822。
