对地热泉周围土壤苔藓中细菌和古菌群落的宏基因组分析。
Metagenomic analysis of bacterial and archaeal assemblages in the soil-mousse surrounding a geothermal spring.
作者信息
Bhatia Sonu, Batra Navneet, Pathak Ashish, Joshi Amit, Souza Leila, Almeida Paulo, Chauhan Ashvini
机构信息
Department of Biotechnology, GGDSD College, Sector 32-C, Chandigarh, India.
School of the Environment, Environmental Biotechnology and Genomics Lab, 1515 S. MLK Blvd., Suite 305B, Building FSHSRC, Florida Agricultural and Mechanical University, Tallahassee, FL 32307, USA.
出版信息
Genom Data. 2015 May 30;5:195-200. doi: 10.1016/j.gdata.2015.05.016. eCollection 2015 Sep.
The soil-mousse surrounding a geothermal spring was analyzed for bacterial and archaeal diversity using 16S rRNA gene amplicon metagenomic sequencing which revealed the presence of 18 bacterial phyla distributed across 109 families and 219 genera. Firmicutes, Actinobacteria, and the Deinococcus-Thermus group were the predominant bacterial assemblages with Crenarchaeota and Thaumarchaeota as the main archaeal assemblages in this largely understudied geothermal habitat. Several metagenome sequences remained taxonomically unassigned suggesting the presence of a repertoire of hitherto undescribed microbes in this geothermal soil-mousse econiche.
利用16S rRNA基因扩增子宏基因组测序技术，对围绕地热泉的土壤泡沫进行细菌和古菌多样性分析，结果显示存在18个细菌门，分布在109个科和219个属中。厚壁菌门、放线菌门以及嗜热放线菌纲是主要的细菌类群，而奇古菌门和泉古菌门是这个研究较少的地热栖息地中的主要古菌类群。几个宏基因组序列在分类学上仍未确定归属，这表明在这个地热土壤泡沫生态小生境中存在一系列迄今未被描述的微生物。
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