Huat Tee Jong, Khan Amir Ali, Abdullah Jafri Malin, Idris Fauziah Mohamad, Jaafar Hasnan
Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia ; Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia.
Department of Neurosciences, School of Medical Sciences, Universiti Sains Malaysia, Jalan Raja Perempuan Zainab II, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia ; Department of Applied Biology, College of Sciences, University of Sharjah, Emirates of Sharjah, P.O. Box 27272, United Arab Emirates ; Sharjah Institute for Medical Research, University of Sharjah, Emirates of Sharjah, P.O. Box 27272, United Arab Emirates.
Genom Data. 2015 Jun 16;5:201-5. doi: 10.1016/j.gdata.2015.06.015. eCollection 2015 Sep.
Recently there has been growing interest in the differentiation of mesenchymal stem cells (MSCs) into neural lineages. Research suggests that MSCs can be differentiated into neural progenitor-like cells (NPCs) under the specific influence of paracrine factors particularly epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF). Our recent research has found that the addition of insulin-like growth factor 1 (IGF-1) with the combination of the EGF and bFGF could significantly improve the growth and survivability of MSC-derived NPCs. To unravel the molecular mechanism of the improved differentiation we compared the microRNA expression profiles of the differentiation under various combinations of growth factors. MSCs were differentiated into neural lineage in 3 groups; Group A (EGF + bFGF), Group B (EGF + bFGF + IGF-1), and Group C (without growth factor). Regulated microRNAs during the early differentiation were identified by detailed microRNA profiling using Affymetrix GeneChip version 2.0 at three time intervals (day 1, day 3 and day 5). The data were deposited in the Gene Expression Omnibus, series GSE60060.
最近，间充质干细胞（MSCs）向神经谱系分化的研究越来越受到关注。研究表明，在旁分泌因子尤其是表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的特定影响下，MSCs可以分化为神经祖细胞样细胞（NPCs）。我们最近的研究发现，添加胰岛素样生长因子1（IGF-1）并结合EGF和bFGF能够显著提高源自MSCs的NPCs的生长和存活率。为了阐明这种改善分化的分子机制，我们比较了在不同生长因子组合下分化过程中的微小RNA表达谱。将MSCs分为3组向神经谱系分化；A组（EGF + bFGF），B组（EGF + bFGF + IGF-1），C组（无生长因子）。在三个时间间隔（第1天、第3天和第5天）使用Affymetrix GeneChip 2.0版通过详细的微小RNA分析鉴定早期分化过程中受调控的微小RNA。数据存于基因表达综合数据库，系列号GSE60060。
