Bloch Katarzyna M, Evans Andrew, Lock Edward A
Liverpool John Moores University, Liverpool, UK.
Genom Data. 2015 Jun 1;5:254-6. doi: 10.1016/j.gdata.2015.05.028. eCollection 2015 Sep.
Aristolochic acids (AAs) are the active components of herbal drugs derived from Aristolochia species that have been used for medicinal purposes since antiquity. However, AAs have recently been discovered to be highly nephrotoxic and induced urothelial cancer in humans and malignant tumors in the kidney and urinary tract of rodents. In this study, we exposed rat renal proximal tubule cells in vitro to a sub-cytotoxic level of AAs at three different time points (6 h, 24 h and 72 h). We then analyzed the gene expression profile after the compound exposure. Functional analysis with Ingenuity Pathways Analysis and DAVID tools revealed that at the late time point (72 h) there are many significantly altered genes involved in cancer-related pathways such as p53 signaling. MIAMI-compliant microarray data are deposited in the NCBI GEO database under accession number GSE68687 and can be found at: http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68687.
马兜铃酸（AAs）是源自马兜铃属植物的草药中的活性成分，自古以来就被用于药用。然而，最近发现AAs具有高度肾毒性，并可导致人类尿路癌以及啮齿动物肾脏和尿路的恶性肿瘤。在本研究中，我们在体外将大鼠肾近端小管细胞暴露于亚细胞毒性水平的AAs中三个不同时间点（6小时、24小时和72小时）。然后我们分析了化合物暴露后的基因表达谱。使用Ingenuity Pathways Analysis和DAVID工具进行的功能分析显示，在较晚时间点（72小时），有许多参与癌症相关途径（如p53信号通路）的基因发生了显著改变。符合MIAMI标准的微阵列数据已存入NCBI GEO数据库，登录号为GSE68687，可在以下网址找到：http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68687 。
