Schmeiser H H, Pool B L, Wiessler M
Cancer Lett. 1984 May;23(1):97-101. doi: 10.1016/0304-3835(84)90067-3.
One of the 2 main components of the commercially available carcinogenic aristolochic acid (AA) was isolated, the other was enriched. Three different aristolochic acid samples (AAI 99% pure; AAI 65% + AAII 35%; AAI 32% + AAII 68%) were assayed for mutagenic activity in Salmonella typhimurium TA1537, TA100 and TA100 NR with and without the addition of a metabolizing mixture. The two main components (AAI and AAII) were direct mutagens in Salmonella strains TA1537 and TA100 with almost equal mutagenic potency. In TA100 NR the aristolochic acid samples showed no or only a very low level of biological activity, indicating the necessity of nitroreduction for the bioactivation of the samples. These findings suggest that both AAI as well as AAII can be used in further studies to elucidate the metabolism of aristolochic acid.
从市售致癌性马兜铃酸（AA）的两种主要成分中分离出一种，另一种进行了富集。对三种不同的马兜铃酸样品（AAI纯度99%；AAI 65% + AAII 35%；AAI 32% + AAII 68%）在添加和不添加代谢混合物的情况下，于鼠伤寒沙门氏菌TA1537、TA100和TA100 NR中检测其致突变活性。这两种主要成分（AAI和AAII）在沙门氏菌菌株TA1537和TA100中是直接诱变剂，诱变效力几乎相等。在TA100 NR中，马兜铃酸样品未显示出生物活性或仅显示出非常低水平的生物活性，这表明样品的生物活化需要硝基还原。这些发现表明，AAI和AAII均可用于进一步研究以阐明马兜铃酸的代谢。
