Bashir Amir, Bisht Balwant Singh, Mir Javaid Iqbal, Patiyal Rabindar Singh, Kumar Rohit
a Department of Zoology , Badshahithaul Campus, HNB Garhwal University , Tehri Garhwal , Uttarakhand , India and.
b Directorate of Coldwater Fisheries Research, Anusandhan Bhawan , Bhimtal , Uttarakhand , India.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4492-4497. doi: 10.3109/19401736.2015.1101537. Epub 2015 Oct 21.
There is a significant taxonomic ambiguity among snow trout species due to their morphometric similarities. In view of this, a morphometric and molecular study was conducted on five different species of genus Schizothorax that have been reported from Kashmir valley. Morphometric data analyzed using multivariate statistics (Principal component analysis and cluster analysis) indicated the significant grouping of species to individual clusters. Mitochondrial DNA cytochrome oxidase I (COI) gene analysis revealed 0.2%-4.5% genetic divergence among the five species. This study confirms that utility of cytochrome oxidase I in species delineation along with morphometric data. Phylogenetic tree obtained using Neighbor-Joining method revealed that all the five species represented distinct species group. The Schizothorax genus formed two distinct clades; one containing S. niger, S. curvifrons and S. plagiostomus, while other clade containing S. esocinus and S. labiatus. This phlogeny trend was also supported by cluster analysis of morphometric characters. The phylogenetic analysis with other published COI sequences revealed distinct nature of these five species. The study may aid in the taxonomic identification of snow trout species in India. This may further increase the knowledge of the ichthyologists in planning conservation and management strategies for these important fish species along with their natural habitat.
由于形态测量学上的相似性，雪 trout 物种之间存在显著的分类学模糊性。鉴于此，对克什米尔山谷报道的裂腹鱼属五个不同物种进行了形态测量学和分子研究。使用多元统计（主成分分析和聚类分析）分析的形态测量数据表明，物种显著地分组到各个聚类中。线粒体 DNA 细胞色素氧化酶 I（COI）基因分析显示，这五个物种之间的遗传差异为 0.2%-4.5%。这项研究证实了细胞色素氧化酶 I 在物种划分以及形态测量数据方面的实用性。使用邻接法获得的系统发育树表明，所有五个物种代表了不同的物种组。裂腹鱼属形成了两个不同的进化枝；一个包含黑裂腹鱼、弯口裂腹鱼和平口裂腹鱼，而另一个进化枝包含似鲮裂腹鱼和唇裂腹鱼。这种系统发育趋势也得到了形态测量特征聚类分析的支持。与其他已发表的 COI 序列进行的系统发育分析揭示了这五个物种的独特性质。该研究可能有助于印度雪 trout 物种的分类鉴定。这可能进一步增加鱼类学家在为这些重要鱼类物种及其自然栖息地规划保护和管理策略方面的知识。
