Samira Jbahi, Saoudi Monji, Abdelmajid Kabir, Hassane Oudadesse, Treq Rebai, Hafed Efeki, Abdelfatteh Elfeki, Hassib Keskes
Campus de Beaulieu, UMR CNRS 6226, University of Rennes, Rennes, France.
Animal Ecophysiology Laboratory, Department of Life Sciences, Sfax Faculty of Science, University of Sfax, Sfax, Tunisia.
Libyan J Med. 2015 Oct 19;10(1):28572. doi: 10.3402/ljm.v10.28572. eCollection 2015.
Synthetic bone graft substitutes such as bioactive glass (BG) material are developed in order to achieve successful bone regeneration. Zn plays an important role in the proper bone growth, development, and maintenance of healthy bones.
This study aims to evaluate in vivo the performance therapy of zinc-doped bioactive glass (BG-Zn) and its applications in biomedicine.
Female Wistar rats were ovariectomized. BG and BG-Zn were implanted in the femoral condyles of Wistar rats and compared to that of control group. Grafted bone tissues were carefully removed to evaluate the oxidative stress status, histomorphometric profile, mechanical property, and mineral bone distribution by using inductively coupled plasma optical emission spectrometry.
A significant decrease of thiobarbituric acid-reactive substances was observed after BG-Zn implantation. Superoxide dismutase, catalase (CAT), and glutathione peroxidase (GPx) activities significantly increased in ovariectomized group implanted with Zinc-doped bioactive glass (OVX-BG-Zn) as compared to ovariectomized group implanted with bioactive glass (OVX-BG). An improved mechanical property was noticed in contact of OVX-BG-Zn (39±6 HV) when compared with that of OVX-BG group (26±9 HV). After 90 days of implantation, the histomorphometric analysis showed that trabecular thickness (Tb.Th) and trabecular number (Tb.N) were significantly increased with 28 and 24%, respectively, in treated rats of OVX-BG-Zn group as compared to those of OVX-BG groups. Trabecular separation (Tb.Sp) and trabecular bone pattern factor (TBPf) were significantly decreased in OVX-BG-Zn group with 29.5 and 54% when compared with those of OVX-BG rat groups. On the other hand, a rise in Ca and P ion concentrations in the implanted microenvironment was shown and lead to the formation/deposition of Ca-P phases. The ratio of pyridinoline [Pyr] to dihydroxylysinonorleucine [DHLNL] cross-links was normalized to the control level.
Our findings suggested that BG-Zn might have promising potential applications for osteoporosis therapy.
为实现成功的骨再生，人们研发了诸如生物活性玻璃（BG）材料等合成骨移植替代物。锌在骨骼的正常生长、发育及维持健康骨骼方面发挥着重要作用。
本研究旨在体内评估掺锌生物活性玻璃（BG-Zn）的治疗性能及其在生物医学中的应用。
对雌性Wistar大鼠进行卵巢切除。将BG和BG-Zn植入Wistar大鼠的股骨髁，并与对照组进行比较。小心取出移植的骨组织，通过电感耦合等离子体发射光谱法评估氧化应激状态、组织形态计量学特征、力学性能和矿化骨分布。
植入BG-Zn后，硫代巴比妥酸反应性物质显著减少。与植入生物活性玻璃的去卵巢组（OVX-BG）相比，植入掺锌生物活性玻璃的去卵巢组（OVX-BG-Zn）中超氧化物歧化酶、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）活性显著增加。与OVX-BG组（26±9 HV）相比，OVX-BG-Zn组（39±6 HV）的力学性能有所改善。植入90天后，组织形态计量学分析表明，与OVX-BG组相比，OVX-BG-Zn组治疗大鼠的小梁厚度（Tb.Th）和小梁数量（Tb.N）分别显著增加28%和24%。与OVX-BG大鼠组相比，OVX-BG-Zn组的小梁间距（Tb.Sp）和小梁骨模式因子（TBPf）分别显著降低29.5%和54%。另一方面，植入微环境中钙和磷离子浓度升高，并导致钙磷相的形成/沉积。吡啶啉[Pyr]与二羟基赖氨酰正亮氨酸[DHLNL]交联的比例恢复至对照水平。
我们的研究结果表明，BG-Zn在骨质疏松症治疗中可能具有广阔的潜在应用前景。
