Su Wen-Ta, Wu Pai-Shuen, Huang Te-Yang
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2015 Jan;46:427-34. doi: 10.1016/j.msec.2014.10.076. Epub 2014 Oct 30.
Mimicking the architecture of the extracellular matrix is an effective strategy for tissue engineering. Composite nanofibers similar to natural bone structure can be prepared via an electrospinning technique and used in biomedical applications. Stem cells from human exfoliated deciduous teeth (SHEDs) can differentiate into multiple cell lineages, such as cells that are alternative sources of stem cells for tissue engineering. Strontium has important functions in bone remodeling; for example, this element can simulate bone formation and decrease bone resorption. Incorporating strontium phosphate into nanofibers provides a potential material for bone tissue engineering. This study investigated the potential of poly(ε-caprolactone) (PCL) nanofibers coated or blended with strontium phosphate for the osteogenic differentiation of SHEDs. Cellular morphology and MTT assay revealed that nanofibers effectively support cellular attachment, spreading, and proliferation. Strontium-loaded PCL nanofibers exhibited higher expressions of collagen type I, alkaline phosphatase, biomineralization, and bone-related genes than pure PCL nanofibers during the osteogenic differentiation of SHEDs. This study demonstrated that strontium can be an effective inducer of osteogenesis for SHEDs. Understanding the function of bioceramics (such as strontium) is useful in designing and developing strategies for bone tissue engineering.
模仿细胞外基质的结构是组织工程的一种有效策略。通过静电纺丝技术可以制备出类似于天然骨结构的复合纳米纤维，并将其用于生物医学应用。人脱落乳牙干细胞（SHEDs）可以分化为多种细胞谱系，例如可作为组织工程干细胞替代来源的细胞。锶在骨重塑中具有重要作用；例如，该元素可以模拟骨形成并减少骨吸收。将磷酸锶掺入纳米纤维中为骨组织工程提供了一种潜在材料。本研究调查了涂覆或混合有磷酸锶的聚（ε-己内酯）（PCL）纳米纤维对SHEDs成骨分化的潜力。细胞形态学和MTT分析表明，纳米纤维有效地支持细胞附着、铺展和增殖。在SHEDs的成骨分化过程中，负载锶的PCL纳米纤维比纯PCL纳米纤维表现出更高的I型胶原蛋白、碱性磷酸酶、生物矿化和骨相关基因表达。本研究表明，锶可以成为SHEDs成骨的有效诱导剂。了解生物陶瓷（如锶）的功能有助于设计和开发骨组织工程策略。
