Suppiger Angela, Aguilar Claudio, Eberl Leo
Department of Microbiology, Institute of Plant Biology, University of Zurich, Zurich, Switzerland.
Environ Microbiol Rep. 2016 Feb;8(1):38-44. doi: 10.1111/1758-2229.12348. Epub 2016 Jan 7.
Many bacteria employ cis-2-unsaturated fatty acids, referred to as DSF (diffusible signal factor) family signals, to communicate with each other. Such systems have been shown to control biofilm formation, motility, production of hydrolytic enzymes and expression of virulence factors. We report the construction of novel biosensors on the basis of components of the Burkholderia-DSF (BDSF) dependent circuitry of Burkholderia cenocepacia H111 and evaluated their utility for detecting the production of DSF family signal molecules. We show that a luxAB-based biosensor responds to nM levels of synthetic BDSF and is suitable to detect a wide range of cis-2 fatty acid molecules. Using this biosensor we show that the production of DSF family molecules is widespread among members of the B. cepacia complex and demonstrate for the first time that DSF-based molecules are also produced by plant-associated Burkholderia species.
许多细菌利用顺式-2-不饱和脂肪酸，即所谓的DSF（可扩散信号因子）家族信号，来相互交流。此类系统已被证明可控制生物膜形成、运动性、水解酶的产生以及毒力因子的表达。我们报告了基于洋葱伯克霍尔德菌H111的伯克霍尔德菌-DSF（BDSF）依赖电路组件构建新型生物传感器，并评估了它们在检测DSF家族信号分子产生方面的效用。我们表明，基于luxAB的生物传感器对纳摩尔水平的合成BDSF有反应，适用于检测广泛的顺式-2脂肪酸分子。使用这种生物传感器，我们表明DSF家族分子的产生在洋葱伯克霍尔德菌复合体成员中很普遍，并首次证明与植物相关的伯克霍尔德菌物种也会产生基于DSF的分子。
