Anniko M, Sjöström B, Thornell L E, Virtanen I
Department of Oto-Rhino-Laryngology and Head & Neck Surgery, University Hospital, Umeå, Sweden.
Acta Otolaryngol. 1989 Mar-Apr;107(3-4):191-201. doi: 10.3109/00016488909127498.
The expression of the five main groups of intermediate filaments (IF) and their subgroups, especially cytokeratins, was analysed in cryosections of the labyrinth of the jerker mouse mutant and compared with normal CBA/CBA controls. Fourteen different well characterized monoclonal antibodies were used. In principle the same pattern of IF was found in the two mouse strains. IF were not found in hair cells of the mouse inner ear, which may reflect a disparity as compared with human fetal hair cells. Chain-specific (epitope-specific) differences were visualized in cytokeratin no. 8 with regard to cells in epithelia involved in the homeostasis of inner ear fluids. Differences in immuno-staining occurred between adjacent cells in the same epithelium, for instance in the semicircular canals.
对颤搐小鼠突变体迷路的冰冻切片中五种主要中间丝（IF）及其亚组，特别是细胞角蛋白的表达进行了分析，并与正常CBA/CBA对照进行了比较。使用了十四种特征明确的不同单克隆抗体。原则上在两种小鼠品系中发现了相同的IF模式。在小鼠内耳的毛细胞中未发现IF，这可能反映了与人类胎儿毛细胞相比的差异。在内耳液体稳态所涉及的上皮细胞中，细胞角蛋白8出现了链特异性（表位特异性）差异。同一上皮中的相邻细胞之间出现了免疫染色差异，例如在半规管中。
