Wikström S O, Anniko M, Thornell L E, Virtanen I
Department of Otolaryngology, Karolinska Sjukhuset, Stockholm, Sweden.
Acta Otolaryngol. 1988 Jul-Aug;106(1-2):71-80. doi: 10.3109/00016488809107373.
The expression of vimentin, cytokeratins (CKs) and neurofilament (NF) proteins was analysed (using monoclonal antibodies) in the mouse inner ear at the otocyst stage (13th gestational day), when organogenesis was largely completed (16th gestational day) and at birth (21st gestational day). Co-expression of vimentin and CKs occurred at the otocyst stage. On the 16th gestational day, most epithelial cells lacked immunoreactivity for vimentin and considerable variation in CK positivity was found between different regions of the epithelial lining. At birth, CK positivity was lacking in the developing organ of Corti but was present in other types of epithelium lining the scala media. In the vestibular half of the labyrinth, positivity for CKs was found at the apical surfaces of both sensory cells and supporting cells and in epithelia lining the membranous labyrinth. Vimentin positivity occurred in the greater epithelial ridge of the differentiating organ of Corti. Even at this stage the statoacoustic ganglion comprised two subpopulations of ganglion cells: those staining for NF proteins and those lacking this immunoreactivity. Thus, as the inner ear matures, a pattern of cytoskeletal reorganization occurs that is dependent on developmental stage.
在小鼠内耳的耳泡期（妊娠第13天）、器官发生基本完成时（妊娠第16天）和出生时（妊娠第21天），（使用单克隆抗体）分析了波形蛋白、细胞角蛋白（CKs）和神经丝（NF）蛋白的表达情况。在耳泡期出现了波形蛋白和CKs的共表达。在妊娠第16天，大多数上皮细胞缺乏波形蛋白免疫反应性，并且上皮内衬不同区域之间的CK阳性存在相当大的差异。出生时，发育中的柯蒂器缺乏CK阳性，但在中阶内衬的其他类型上皮中存在。在迷路的前庭部分，在感觉细胞和支持细胞的顶端表面以及膜迷路内衬的上皮中发现了CK阳性。波形蛋白阳性出现在分化中的柯蒂器的大上皮嵴中。即使在这个阶段，听神经节也由两个神经节细胞亚群组成：那些对NF蛋白染色的细胞和那些缺乏这种免疫反应性的细胞。因此，随着内耳成熟，会发生一种依赖于发育阶段的细胞骨架重组模式。
