Anniko M, Thornell L E, Gustavsson H, Virtanen I
ORL J Otorhinolaryngol Relat Spec. 1986;48(2):98-106. doi: 10.1159/000275854.
The presence of intermediate filaments in the inner ear of the newborn mouse was analyzed with immunofluorescence techniques using antibodies against the five classes of intermediate filaments: cytokeratins, vimentin, desmin, neurofilaments and glial fibrillary acid protein (GFA). Neurofilaments were found in all nerve fibers from the ganglion cell to the hair cell. In the vestibular ganglion two subpopulations of ganglion cells were identified: a minor part staining intensively with neurofilament and the major part of cells lacking this immunofluorescence. Vimentin occurred in a number of supporting structures in the membranous labyrinth, but not in vestibular or cochlear ganglion cells. Cytokeratins, desmin or GFA were not identified in the inner ear.
利用针对五类中间丝的抗体，即细胞角蛋白、波形蛋白、结蛋白、神经丝和胶质纤维酸性蛋白（GFA），采用免疫荧光技术分析了新生小鼠内耳中中间丝的存在情况。从神经节细胞到毛细胞的所有神经纤维中均发现了神经丝。在前庭神经节中，鉴定出了两类神经节细胞亚群：一小部分细胞神经丝染色强烈，而大部分细胞缺乏这种免疫荧光。波形蛋白存在于膜迷路的一些支持结构中，但在前庭或耳蜗神经节细胞中不存在。在内耳中未鉴定出细胞角蛋白、结蛋白或GFA。
