Wahlberg P, Fex G, Wennerberg J, Willén R
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Acta Otolaryngol. 1989 Mar-Apr;107(3-4):313-20. doi: 10.3109/00016488909127515.
The measurement and localisation of cellular retinol-binding protein (CRBP), in samples of normal oral mucosa, larynx papilloma and malignant lymphoma of the oropharynx, was performed with a radioimmunoassay and immunolocalisation techniques. As compared with CRBP concentration in normal mucosa, those in laryngeal papilloma were significantly higher, but those of malignant lymphoma were similar. CRBP concentrations were highest in maturing keratinocytes within the prickle cell layers of normal mucosa and in laryngeal papillomas, as estimated on the basis of immunoreactivity to CRBP. The retinyl palmitate concentrations in extracts of oral mucosa correlated to the retinol concentrations, both in plasma and mucosal extracts, but not to the CRBP content in mucosal extracts. The immunolocalisation of a cellular retinoic acid-binding protein (CRABP) like antigen in normal oral mucosa showed much the same picture with strongest staining of the maturing keratinocytes of the prickle cell layers.
采用放射免疫分析法和免疫定位技术，对正常口腔黏膜、喉乳头状瘤和口咽恶性淋巴瘤样本中的细胞视黄醇结合蛋白（CRBP）进行了测量和定位。与正常黏膜中的CRBP浓度相比，喉乳头状瘤中的CRBP浓度显著更高，但恶性淋巴瘤中的CRBP浓度与之相似。根据对CRBP的免疫反应性估计，正常黏膜和喉乳头状瘤棘细胞层内成熟角质形成细胞中的CRBP浓度最高。口腔黏膜提取物中的棕榈酸视黄酯浓度与血浆和黏膜提取物中的视黄醇浓度相关，但与黏膜提取物中的CRBP含量无关。正常口腔黏膜中一种细胞视黄酸结合蛋白（CRABP）样抗原的免疫定位显示，棘细胞层成熟角质形成细胞染色最强，情况大致相同。
