Wahlberg P, Fex G, Biörklund A, Tropé C, Willén R
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Int J Cancer. 1988 May 15;41(5):771-6. doi: 10.1002/ijc.2910410523.
The concentration of cellular retinol-binding protein (CRBP) was determined by radioimmunoassay in biopsies of normal mucosa and squamous-cell carcinomas of cervix uteri from 30 women. The tumour tissues contained significantly higher concentrations of CRBP (median = 120 micrograms/g protein) than normal mucosa (median = 32 micrograms/g protein). The distribution of CRBP in normal mucosa and squamous-cell carcinomas from cervix uteri and from oral cavity was evaluated by immunohistochemical techniques. In tissue sections of normal epithelium from the cervix uteri and the oral cavity, the maturing keratinocytes in the prickle-cell layers were moderately or strongly stained when antiserum against CRBP was used, while the proliferating cells in the basal-cell layer were stained only lightly if at all. Squamous-cell carcinomas of the cervix uteri and the oral cavity presented much the same picture. The observed difference in CRBP concentration between squamous-cell carcinomas and normal squamous-cell mucosa may therefore be more quantitative than qualitative.
采用放射免疫分析法测定了30名女性子宫颈正常黏膜和鳞状细胞癌活检组织中细胞视黄醇结合蛋白（CRBP）的浓度。肿瘤组织中CRBP的浓度（中位数=120微克/克蛋白质）显著高于正常黏膜（中位数=32微克/克蛋白质）。采用免疫组织化学技术评估了CRBP在子宫颈和口腔的正常黏膜及鳞状细胞癌中的分布。在子宫颈和口腔正常上皮的组织切片中，当使用抗CRBP抗血清时，棘细胞层中成熟的角质形成细胞呈中度或强染色，而基底层中的增殖细胞即使染色也很淡。子宫颈和口腔的鳞状细胞癌呈现出大致相同的情况。因此，鳞状细胞癌与正常鳞状黏膜之间观察到的CRBP浓度差异可能更多是数量上的而非质量上的。
