组织蛋白酶E的高表达与慢性阻塞性肺疾病患者气流受限的严重程度相关。

High Expression of Cathepsin E is Associated with the Severity of Airflow Limitation in Patients with COPD.

作者信息

Cao Wei-Jun, Li Man-Hui, Li Jian-Xiong, Xu Xin, Ren Sheng-Xiang, Rajbanshi Bhavana, Xu Jin-Fu

机构信息

a 1 Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine , Shanghai , China.

b 2 Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, Soochow University , Suzhou , China.

出版信息

COPD. 2016;13(2):160-6. doi: 10.3109/15412555.2015.1057273. Epub 2015 Oct 21.

DOI:10.3109/15412555.2015.1057273

PMID:26488201

Abstract

BACKGROUND

It was reported that Cathepsin E (Cat E) plays a critical role in antigen processing and in the development of pulmonary emphysema. The aim of this study was to investigate the role of Cat E and airflow limitation in the pathogenesis of COPD.

METHODS

Sixty-five patients with COPD, 20 smoking control subjects without COPD and 15 non-smoking healthy control subjects were enrolled. Cat E and EIC (Elastase inhibitory capacity) expressions were measured by ELISA in sputum and serum samples and compared according to different subgroups.

RESULTS

Cat E concentrations were significantly higher in patients with COPD than smoking control and non-smoking control subjects (P < 0.01). The levels of CatE were inversely correlated with FEV1% predicted in COPD patients (r = -0.95, P < 0.01). The levels of EIC were inversely positively correlated with FEV1% predicted in COPD patients (r = 0.926, P < 0.01). Levels of Cat E were also inversely correlated with the levels of EIC (r = -0.922, P < 0.01).

CONCLUSIONS

Cat E contributes to the severity of airflow limitation during progression of COPD.

摘要

背景

据报道，组织蛋白酶E（Cat E）在抗原加工和肺气肿的发展过程中起关键作用。本研究的目的是探讨Cat E和气流受限在慢性阻塞性肺疾病（COPD）发病机制中的作用。

方法

招募了65例COPD患者、20例无COPD的吸烟对照者和15例不吸烟的健康对照者。通过酶联免疫吸附测定（ELISA）法检测痰液和血清样本中Cat E和弹性蛋白酶抑制能力（EIC）的表达，并根据不同亚组进行比较。

结果

COPD患者的Cat E浓度显著高于吸烟对照者和不吸烟对照者（P < 0.01）。COPD患者中Cat E水平与预测的第一秒用力呼气容积百分比（FEV1%）呈负相关（r = -0.95，P < 0.01）。COPD患者中EIC水平与预测的FEV1%呈正相关（r = 0.926，P < 0.01）。Cat E水平也与EIC水平呈负相关（r = -0.922，P < 0.01）。