Gong Han Yu, Li Yang, Fang Gen, Hu Dao Heng, Jin Wen Bin, Wang Zhao Hai, Li Yang Sheng
State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China; Engineering Research Centre for the Protection and Utilization of Bioresource in Ethnic Area of Southern China, South-Central University for Nationalities, Wuhan, China.
State Key Laboratory for Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, China.
PLoS One. 2015 Oct 21;10(10):e0140928. doi: 10.1371/journal.pone.0140928. eCollection 2015.
To find a way to promote the rate of carbon flux and further improve the photosynthetic rate in rice, two CO2-transporting and fixing relevant genes, Ictb and FBP/Sbpase, which were derived from cyanobacteria with the 35SCaMV promotor in the respective constructs, were transformed into rice. Three homologous transgenic groups with Ictb, FBP/Sbpase and the two genes combined were constructed in parallel, and the functional effects of these two genes were investigated by physiological, biochemical and leaf anatomy analyses. The results indicated that the mesophyll conductance and net photosynthetic rate were higher at approximately 10.5-36.8% and 13.5-34.6%, respectively, in the three groups but without any changes in leaf anatomy structure compared with wild type. Other physiological and biochemical parameters increased with the same trend in the three groups, which showed that the effect of FBP/SBPase on improving photosynthetic capacity was better than that of ICTB and that there was an additive effect in ICTB+FBP/SBPase. ICTB localized in the cytoplasm, whereas FBP/SBPase was successfully transported to the chloroplast. The two genes might show a synergistic interaction to promote carbon flow and the assimilation rate as a whole. The multigene transformation engineering and its potential utility for improving the photosynthetic capacity and yield in rice were discussed.
为找到提高水稻碳通量速率并进一步提升光合速率的方法，将两个与二氧化碳运输和固定相关的基因，即来自蓝细菌的Ictb和FBP/Sbpase，分别构建在带有35SCaMV启动子的载体中，并转化到水稻中。平行构建了三个同源转基因组，分别含有Ictb、FBP/Sbpase以及这两个基因的组合，通过生理、生化和叶片解剖分析来研究这两个基因的功能效应。结果表明，与野生型相比，这三个组的叶肉导度和净光合速率分别提高了约10.5 - 36.8%和13.5 - 34.6%，但叶片解剖结构没有任何变化。其他生理和生化参数在这三个组中也呈相同趋势增加，这表明FBP/SBPase对提高光合能力的效果优于ICTB，且ICTB + FBP/SBPase存在累加效应。ICTB定位于细胞质中，而FBP/SBPase成功转运到叶绿体中。这两个基因可能表现出协同相互作用，以整体促进碳流和同化速率。讨论了多基因转化工程及其在提高水稻光合能力和产量方面的潜在应用。
