Shehata M G, Kenawy M A, el Said S M, Beier J C, Gwadz R, Shaaban M
Research and Training Center on Vectors of Diseases, Ain Shams University, Abbassia, Cairo, Egypt.
Ann Parasitol Hum Comp. 1989;64(1):72-6. doi: 10.1051/parasite/198964172.
Two immunoassays for malaria sporozoite detection and identification, the immunoradiometric assay (IRMA) and the enzyme-linked immunosorbent assay (ELISA) using the species-specific monoclonal antibodies are routinely performed in our laboratory. We analyzed (573) anopheline mosquitoes of A. sergenti (463), A. pharoensis (81) and A. multicolor (29) collected from Siwa-oases and Faiyum Governorate (two known active malaria foci in Egypt), for detection of P. falciparum and P. vivax sporozoites. P. falciparum sporozoites were detected by both IRMA and ELISA tests in two A. sergenti mosquitoes (one from Siwa 1/389 = (0.26%) and one from Faiyum Governorate 1/74 = (1.35%)). No P. vivax sporozoites were detected. This finding is important in explaining the malaria transmission and provide first incrimination of An. sergenti as the responsible vector of malaria in Siwa-oasis, Egypt.
我们实验室常规采用两种用于疟原虫检测和鉴定的免疫测定法，即免疫放射测定法（IRMA）和使用种特异性单克隆抗体的酶联免疫吸附测定法（ELISA）。我们分析了从锡瓦绿洲和法尤姆省（埃及两个已知的活跃疟疾疫源地）采集的573只按蚊，其中包括463只塞内加尔按蚊、81只法老按蚊和29只多彩按蚊，以检测恶性疟原虫和间日疟原虫的子孢子。通过IRMA和ELISA检测，在两只塞内加尔按蚊中检测到了恶性疟原虫子孢子（一只来自锡瓦 1/389 =（0.26%），一只来自法尤姆省 1/74 =（1.35%））。未检测到间日疟原虫子孢子。这一发现对于解释疟疾传播具有重要意义，并首次认定塞内加尔按蚊是埃及锡瓦绿洲疟疾传播的责任媒介。
