Prim Denis, Pfeifer Marc E
Institute of Life Technologies, University of Applied Sciences and Arts Western Switzerland, Route du Rawyl 64, Sion 2, CH-1950, Switzerland.
Methods Mol Biol. 2016;1352:157-66. doi: 10.1007/978-1-4939-3037-1_12.
Immobilization of peptides to a solid surface is frequently an important first step before they can be probed with a variety of biological samples in a heterogeneous assay format for research and clinical diagnostic purposes. Peptides can be derivatized in many ways to subsequently covalently attach them to an activated solid surface such as epoxy-functionalized glass slides. Here, we describe a clean, efficient, and reproducible fabrication process based on catalyst-free click chemistry compatible with the construction of low- to high-density peptide microarrays.
在使用各种生物样品以异质分析形式进行研究和临床诊断之前，将肽固定到固体表面通常是重要的第一步。肽可以通过多种方式衍生化，随后将它们共价连接到活化的固体表面，如环氧官能化的载玻片。在这里，我们描述了一种基于无催化剂点击化学的清洁、高效且可重复的制备过程，该过程与构建低密度至高密度肽微阵列兼容。
