Vutti Surendra, Schoffelen Sanne, Bolinsson Jessica, Buch-Månson Nina, Bovet Nicolas, Nygård Jesper, Martinez Karen L, Meldal Morten
Center for Evolutionary Chemical Biology, Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
Nano-science Center and Center for Quantum Devices, Niels Bohr Institute, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen (Denmark).
Chemistry. 2016 Jan 11;22(2):496-500. doi: 10.1002/chem.201504540. Epub 2015 Dec 8.
Semiconductor nanowires (NWs) are gaining significant importance in various biological applications, such as biosensing and drug delivery. Efficient and controlled immobilization of biomolecules on the NW surface is crucial for many of these applications. Here, we present for the first time the use of the Cu(I) -catalyzed alkyne-azide cycloaddition and its strain-promoted variant for the covalent functionalization of vertical NWs with peptides and proteins. The potential of the approach was demonstrated in two complementary applications of measuring enzyme activity and protein binding, which is of general interest for biological studies. The attachment of a peptide substrate provided NW arrays for the detection of protease activity. In addition, green fluorescent protein was immobilized in a site-specific manner and recognized by antibody binding to demonstrate the proof-of-concept for the use of covalently modified NWs for diagnostic purposes using minute amounts of material.
半导体纳米线（NWs）在各种生物应用中，如生物传感和药物递送，正变得越来越重要。生物分子在NW表面的高效且可控的固定化对于许多此类应用至关重要。在此，我们首次展示了使用铜（I）催化的炔烃 - 叠氮环加成反应及其应变促进变体，用于垂直NWs与肽和蛋白质的共价功能化。该方法的潜力在测量酶活性和蛋白质结合这两个互补的应用中得到了证明，这对于生物学研究具有普遍意义。肽底物的附着为蛋白酶活性的检测提供了NW阵列。此外，绿色荧光蛋白以位点特异性方式固定，并通过抗体结合得以识别，从而证明了使用共价修饰的NWs以微量材料用于诊断目的的概念验证。
