Usui Kenji, Tomizaki Kin-ya, Mihara Hisakazu
FIRST (Faculty of Frontiers of Innovative Research in Science and Technology), Konan University, 7-1-20 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan.
Department of Materials Chemistry, Ryukoku University, Seta-Oe, Otsu, 520-2194, Japan.
Methods Mol Biol. 2016;1352:199-210. doi: 10.1007/978-1-4939-3037-1_15.
There has been increasing interest in the potential use of microarray technologies to perform systematic and high-throughput cell-based assays. We are currently focused on developing more practical array formats and detection methods that will enable researchers to conduct more detailed analyses in cell microarray studies. In this chapter, we describe the construction of a novel peptide-array format system for analyzing cellular toxicity and cellular uptake. In this system, a peptide is immobilized at the bottom of a conventional 96-well plate using a photo-cleavable linker. The peptide can then be released from the bottom by irradiating the desired wells with UV light, thus allowing the cytotoxicity or cellular uptake of the peptide to be monitored. This system will facilitate the realization of high-throughput cell arrays for cellomics analyses and cell-based phenotypic drug screens.
人们对利用微阵列技术进行系统的、高通量的基于细胞的分析的潜在用途越来越感兴趣。我们目前专注于开发更实用的阵列形式和检测方法，以使研究人员能够在细胞微阵列研究中进行更详细的分析。在本章中，我们描述了一种用于分析细胞毒性和细胞摄取的新型肽阵列形式系统的构建。在该系统中，使用光可裂解接头将肽固定在传统96孔板的底部。然后通过用紫外光照射所需孔，使肽从底部释放，从而能够监测肽的细胞毒性或细胞摄取。该系统将有助于实现用于细胞组学分析和基于细胞的表型药物筛选的高通量细胞阵列。
